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Literature summary extracted from

  • Robertson, D.L.; Alberte, R.S.
    Isolation and characterization of glutamine synthetase from the marine diatom Skeletonema costatum (1996), Plant Physiol., 111, 1169-1175.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
6.3.1.2 isoform GS1 is more labile than isoform GS2 in the early stages of purification Skeletonema costatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 Ala
-
 Skeletonema costatum
6.3.1.2 Gly
-
 Skeletonema costatum
6.3.1.2 L-methionine sulfoximine
-
 Skeletonema costatum
6.3.1.2 Phosphinothricin
-
 Skeletonema costatum
6.3.1.2 Ser
-
 Skeletonema costatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 0.5
-
 ATP
-
 Skeletonema costatum
6.3.1.2 0.7
-
 NH4+
-
 Skeletonema costatum
6.3.1.2 5.7
-
 Glu
-
 Skeletonema costatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 70000
-
 6 * 70000, SDS-PAGE Skeletonema costatum
6.3.1.2 400000
-
 isoform GS2, gel filtration Skeletonema costatum

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Skeletonema costatum
-
 isoform GS1 and GS2
-
6.3.1.2 Skeletonema costatum CCMP 1332
-
 isoform GS1 and GS2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2
-
 Skeletonema costatum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 4.27
-
-
 Skeletonema costatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
 Skeletonema costatum ADP + phosphate + L-Gln
-
 ?
6.3.1.2 ATP + L-Glu + NH4+
-
 Skeletonema costatum CCMP 1332 ADP + phosphate + L-Gln
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 hexamer 6 * 70000, SDS-PAGE Skeletonema costatum