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Literature summary extracted from

  • Rowe, W.B.
    Glutamine synthetase from muscle (1985), Methods Enzymol., 113, 199-212.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 ADP
-
Ovis aries
6.3.1.2 ADP
-
Rattus norvegicus
6.3.1.2 ADP
-
Sus scrofa
6.3.1.2 Ala D-Ala Rattus norvegicus
6.3.1.2 Ca2+ above 2 mM CaCl2, in presence of 20 mM MgCl2 and 10 mM ATP Rattus norvegicus
6.3.1.2 Carbamoyl phosphate
-
Rattus norvegicus
6.3.1.2 D-Methionine sulfone
-
Rattus norvegicus
6.3.1.2 D-Methionine sulfone
-
Sus scrofa
6.3.1.2 diphosphate
-
Rattus norvegicus
6.3.1.2 GDP weak Ovis aries
6.3.1.2 GDP
-
Rattus norvegicus
6.3.1.2 GDP
-
Sus scrofa
6.3.1.2 L-methionine sulfone
-
Rattus norvegicus
6.3.1.2 L-methionine sulfone
-
Sus scrofa
6.3.1.2 L-methionine sulfoximine irreversible at high concentrations, competitive with L-Glu; S,R-sulfoximine and S-sulfoximine inhibit, R-sulfoximine is ineffective Rattus norvegicus
6.3.1.2 L-methionine sulfoximine
-
Sus scrofa
6.3.1.2 Mg2+ inhibition at more than 20 mM excess of MgCl2 over ATP Rattus norvegicus
6.3.1.2 Mn2+ above 2 mM MnCl2, in presence of 20 mM MgCl2 and 10 mM ATP Rattus norvegicus
6.3.1.2 phosphate
-
Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Mg2+ required Rattus norvegicus
6.3.1.2 Mg2+ required Sus scrofa
6.3.1.2 Mg2+ optimal ratio of MgCl2 to ATP is 2:1 at 1 mM ATP, inhibition at more than 20 mM excess of MgCl2 over ATP Rattus norvegicus
6.3.1.2 Mn2+ optimal concentration is 2 mM, 25% of the maximal activity relative to Mg2+ activation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Ovis aries
-
-
-
6.3.1.2 Rattus norvegicus
-
-
-
6.3.1.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2
-
Rattus norvegicus
6.3.1.2
-
Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.2 brain
-
Rattus norvegicus
-
6.3.1.2 brain
-
Sus scrofa
-
6.3.1.2 brain
-
Ovis aries
-
6.3.1.2 heart
-
Sus scrofa
-
6.3.1.2 kidney
-
Rattus norvegicus
-
6.3.1.2 liver
-
Rattus norvegicus
-
6.3.1.2 muscle
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 1
-
-
Sus scrofa
6.3.1.2 1.1
-
-
Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + hydroxylamine
-
Ovis aries ADP + phosphate + gamma-glutamyl hydroxamate
-
?
6.3.1.2 ATP + L-Glu + NH4+
-
Rattus norvegicus ADP + phosphate + L-Gln
-
?
6.3.1.2 ATP + L-Glu + NH4+
-
Sus scrofa ADP + phosphate + L-Gln
-
?
6.3.1.2 ATP + L-Glu + NH4+
-
Ovis aries ADP + phosphate + L-Gln
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 7.2
-
-
Rattus norvegicus