Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kumar, P.S.; Rao, S.L.N.
    Identification and characterization of three forms of glutamine synthetase unique to Rhizobia (1986), Curr. Microbiol., 14, 113-116.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 Ala beta-Ala; inhibition of enzyme form GSII and GSIII Rhizobium phaseoli
6.3.1.2 L-methionine sulfoximine inhibition of enzyme form GSII and GSIII Rhizobium phaseoli
6.3.1.2 L-methionine sulfoximine
-
Sinorhizobium meliloti
6.3.1.2 Mg2+ inhibits enzyme form GSIII to nearly 72%, enzyme form GSI 30%, and enzyme form GSII 33.3% Rhizobium phaseoli
6.3.1.2 thymidine complete inhibition of enzyme form GSII, partial inhibition of enzyme form GSI, no effect on enzyme form GSIII Rhizobium phaseoli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 64570
-
enzyme form GSI, gel filtration Rhizobium phaseoli
6.3.1.2 489800
-
enzyme form GSII, gel filtration Rhizobium phaseoli
6.3.1.2 891000
-
enzyme form GSIII, gel filtration Rhizobium phaseoli

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Rhizobium leguminosarum
-
3 enzyme forms: GSI, GSII and GSIII
-
6.3.1.2 Rhizobium phaseoli
-
-
-
6.3.1.2 Sinorhizobium meliloti
-
3 enzyme forms: GSI, GSII and GSIII
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
Sinorhizobium meliloti ADP + phosphate + L-Gln
-
?
6.3.1.2 ATP + L-Glu + NH4+
-
Rhizobium leguminosarum ADP + phosphate + L-Gln
-
?
6.3.1.2 ATP + L-Glu + NH4+
-
Rhizobium phaseoli ADP + phosphate + L-Gln
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.1.2 60
-
20.5% inactivation of enzyme form GSI, 55.4% inactivation of enzyme form GSII, 27.3% inactivation of enzyme form GSIII Rhizobium phaseoli
6.3.1.2 60
-
15 min, 40% inactivation of enzyme form GSI, 74.6% inactivation of enzyme form GSII, 35.5% inactivation of enzyme form GSIII Rhizobium leguminosarum
6.3.1.2 60
-
15 min, 45.8% inactivation of enzyme form GSI, 61.2% inactivation of enzyme form GSII, 37.5% inactivation of enzyme form GSIII Sinorhizobium meliloti