Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(R)-2-[3-(diethoxyphosphoryl)phenylamino]-3-hydroxypropionic acid | - |
Mycobacterium tuberculosis | |
(S)-2-[3-(diethoxyphosphoryl)phenylamino]-3-hydroxypropionic acid | - |
Mycobacterium tuberculosis | |
(S)-3-hydroxy-2-(3-methylsulfanylphenylamino)propionic acid | - |
Mycobacterium tuberculosis | |
(S)-3-hydroxy-2-(3-sulfamoylphenylamino)propionic acid | - |
Mycobacterium tuberculosis | |
3-((R)-1-carboxy-2-hydroxyethylamino)benzoic acid | - |
Mycobacterium tuberculosis | |
3-((S)-1-carboxy-2-hydroxyethylamino)benzoic acid | - |
Mycobacterium tuberculosis | |
3-(carboxymethylamino)benzoic acid | - |
Mycobacterium tuberculosis |
Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a potential antimycobacterial drug target | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2S)-2-amino-4-(methylsulfonyl)butanoic acid | - |
Mycobacterium tuberculosis | |
(2S,5R)-2,6-diamino-5-hydroxyhexanoic acid | docks at the amino acid binding site of the enzyme, structure, overview | Mycobacterium tuberculosis | |
(3-methanesulfinylphenylamino)acetic acid | 30% inhibition at 1.0 mM | Mycobacterium tuberculosis | |
(R)-3-hydroxy-2-(3-sulfamoylphenylamino)propionic acid | 33% inhibition at 1.0 mM | Mycobacterium tuberculosis | |
(S)-3-hydroxy-2-(3-methanesulfinylphenylamino)propionic acid | 13% inhibition at 1.0 mM | Mycobacterium tuberculosis | |
2-amino-4-(hydroxyamino)butanoic acid | - |
Mycobacterium tuberculosis | |
2-oxo-2,3-dihydro-1H-benzimidazole-5-sulfonamide | 24% inhibition at 1.0 mM | Mycobacterium tuberculosis | |
3-[(1H-1,2,4-triazol-3-ylcarbonyl)amino]benzoic acid | 26% inhibition at 1.0 mM | Mycobacterium tuberculosis | |
L-methionine sulfoximine | - |
Mycobacterium tuberculosis | |
L-methionine-(S)-sulfoximine | docks at the amino acid binding site of the enzyme, structure, overview | Mycobacterium tuberculosis | |
additional information | no inhibition by L-homoserine, (R)-3-hydroxy-2-(3-methanesulfinylphenylamino)propionic acid, (R)-3-hydroxy-2-(3-methylsulfanylphenylamino)propionic acid, (3-sulfamoylphenylamino)acetic acid, (3-methylsulfanylphenylamino)acetic acid, 2-amino-3-(2-phosphonomethylphenyl)propanoic acid, 2-amino-3-(2-aminomethylphenyl)propanoic acid, and methyl 2-amino-3-(2-phosphonomethylphenyl)propanoate, inhibitor screening and docking studies, overview | Mycobacterium tuberculosis | |
N-(4-hydroxy-3-sulfophenyl)glycine | 48% inhibition at 1.0 mM | Mycobacterium tuberculosis | |
Phosphinothricin | - |
Mycobacterium tuberculosis | |
[3-(diethoxyphosphoryl)phenylamino]acetic acid | 42% inhibition at 1.0 mM | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + NH3 | Mycobacterium tuberculosis | glnA1 is essential for Mycobacterium tuberculosis virulence | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH3 | Mycobacterium tuberculosis H37Rv | glnA1 is essential for Mycobacterium tuberculosis virulence | ADP + phosphate + L-glutamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WN39 | gene glnA1 | - |
Mycobacterium tuberculosis H37Rv | P9WN39 | gene glnA1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + NH3 | glnA1 is essential for Mycobacterium tuberculosis virulence | Mycobacterium tuberculosis | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH3 | amino acid binding site and structure, overview | Mycobacterium tuberculosis | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH3 | glnA1 is essential for Mycobacterium tuberculosis virulence | Mycobacterium tuberculosis H37Rv | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH3 | amino acid binding site and structure, overview | Mycobacterium tuberculosis H37Rv | ADP + phosphate + L-glutamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Glutamine synthetase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0019 | - |
pH 7.5, 22°C | Mycobacterium tuberculosis | Phosphinothricin | |
0.026 | - |
pH 7.5, 22°C | Mycobacterium tuberculosis | 2-amino-4-(hydroxyamino)butanoic acid | |
0.033 | - |
pH 7.5, 22°C | Mycobacterium tuberculosis | (2S)-2-amino-4-(methylsulfonyl)butanoic acid | |
0.051 | - |
pH 7.5, 22°C | Mycobacterium tuberculosis | L-methionine-(S)-sulfoximine | |
0.056 | 0.058 | dependent on the solvent, pH 7.5, 22°C | Mycobacterium tuberculosis | (2S,5R)-2,6-diamino-5-hydroxyhexanoic acid | |
0.076 | - |
pH 7.5, 22°C | Mycobacterium tuberculosis | Phosphinothricin | |
0.081 | - |
pH 7.5, 22°C | Mycobacterium tuberculosis | L-methionine sulfoximine |