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Literature summary for 6.3.1.2 extracted from

  • Singh, U.; Panchanadikar, V.; Sarkar, D.
    Development of a simple assay protocol for high-throughput screening of Mycobacterium tuberculosis glutamine synthetase for the identification of novel inhibitors (2005), J. Biomol. Screen., 10, 725-729.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
L-methionine S-sulfoximine IC50: 3 mM Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
ADP pH 7.0 Mycobacterium tuberculosis
8.3
-
hydroxylamine pH 7.0 Mycobacterium tuberculosis
60
-
L-Gln pH 7.0 Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ Km: 0.025 mM Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mycobacterium tuberculosis essential enzyme involved in the pathogenicity of Mycobacterium tuberculosis ?
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Gln + hydroxylamine + ADP
-
Mycobacterium tuberculosis gamma-glutamylhydroxamate + NH4+ + ?
-
?
additional information essential enzyme involved in the pathogenicity of Mycobacterium tuberculosis Mycobacterium tuberculosis ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
pH 7.0, 0.002 mM ADP, 0.005 mM arsenate, 0.025 mM Mn2+, 60 mM L-glutamine, 8.3 mM hydroxylamine, stable for at least 3 h Mycobacterium tuberculosis
37
-
pH 7.0, 0.002 mM ADP, 0.005 mM arsenate, 0.025 mM Mn2+, 60 mM L-glutamine, 8.3 mM hydroxylamine, stable for at least 3 h Mycobacterium tuberculosis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3
-
IC50: 3 mM Mycobacterium tuberculosis L-methionine S-sulfoximine