Cloned (Comment) | Organism |
---|---|
mutant and native enzymes expressed in Escherichia coli ET6017 | Trichormus azollae |
Protein Variants | Comment | Organism |
---|---|---|
D51A | 7% of activity in forward reaction, 4% of activity in reverse reaction no effect on protein structure | Trichormus azollae |
D51E | 5% of activity in forward reaction, 87% of activity in reverse reaction no effect on protein structure | Trichormus azollae |
D51N | 1.7% of activity in forward reaction, 2.4% of activity in reverse reaction no effect on protein structure | Trichormus azollae |
D51R | complete loss of activity, no effect on protein structure | Trichormus azollae |
D51S | 24% of activity in forward reaction, 15% of activity in reverse reaction, no effect on protein structure | Trichormus azollae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-methionine-DL-sulfoximine | MSX, complete inactivation of wild-type and mutant enzymes except D51E mutant, which shows only 10% inhibition | Trichormus azollae | |
Phosphinothricin | PPT, complete inactivation of wild-type and mutant enzymes except D51E mutant, which shows 70% inhibition | Trichormus azollae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
ATP | D51A mutant, pH 7.0, 30°C | Trichormus azollae | |
0.05 | - |
NH4+ | wild type enzyme, pH 7.0, 30°C | Trichormus azollae | |
0.07 | - |
ATP | D51S mutant, pH 7.0, 30°C | Trichormus azollae | |
0.12 | - |
ATP | D51N mutant, pH 7.0, 30°C | Trichormus azollae | |
0.21 | - |
ATP | wild type enzyme, pH 7.0, 30°C | Trichormus azollae | |
0.32 | - |
L-glutamate | D51A mutant, pH 7.0, 30°C | Trichormus azollae | |
0.37 | - |
L-glutamate | D51S mutant, pH 7.0, 30°C | Trichormus azollae | |
0.55 | - |
NH4+ | D51E mutant, pH 7.0, 30°C | Trichormus azollae | |
0.85 | - |
L-glutamate | D51N mutant, pH 7.0, 30°C | Trichormus azollae | |
0.92 | - |
NH4+ | D51N mutant, pH 7.0, 30°C | Trichormus azollae | |
1.5 | - |
L-glutamate | wild type enzyme, pH 7.0, 30°C | Trichormus azollae | |
5.95 | - |
NH4+ | D51S mutant, pH 7.0, 30°C | Trichormus azollae | |
10.6 | - |
ATP | D51E mutant, pH 7.0, 30°C | Trichormus azollae | |
12.45 | - |
NH4+ | D51A mutant, pH 7.0, 30°C | Trichormus azollae | |
16 | - |
L-glutamate | D51E mutant, pH 7.0, 30°C | Trichormus azollae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glutamate + NH4+ | Trichormus azollae | central enzyme of nitrogen metabolism | ADP + phosphate + L-glutamine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichormus azollae | Q9RHZ1 | cyanobacterium | - |
Purification (Comment) | Organism |
---|---|
recombinant enzymes and enzyme from host strain | Trichormus azollae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glutamate + NH4+ | - |
Trichormus azollae | ADP + phosphate + L-glutamine | - |
r | |
ATP + glutamate + NH4+ | central enzyme of nitrogen metabolism | Trichormus azollae | ADP + phosphate + L-glutamine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GlnA | - |
Trichormus azollae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
L-glutamate | D51E mutant, pH 7.0, 30°C | Trichormus azollae | |
2.6 | - |
L-glutamate | D51A mutant, pH 7.0, 30°C | Trichormus azollae | |
13.1 | - |
L-glutamate | D51S mutant, pH 7.0, 30°C | Trichormus azollae | |
42 | - |
L-glutamate | wild type enzyme, pH 7.0, 30°C | Trichormus azollae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
L-methionine-DL-sulfoximine | wild-type enzyme, biosynthetic reaction, pH 7.0, 30°C | Trichormus azollae | |
0.0003 | - |
Phosphinothricin | wild-type enzyme, biosynthetic reaction, pH 7.0, 30°C | Trichormus azollae | |
0.1 | - |
Phosphinothricin | D51E mutant enzyme, biosynthetic reaction, pH 7.0, 30°C | Trichormus azollae | |
2.94 | - |
L-methionine-DL-sulfoximine | D51E mutant enzyme, biosynthetic reaction, pH 7.0, 30°C | Trichormus azollae |