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Literature summary for 6.3.1.2 extracted from

  • Shatters, R.G.; Liu, Y.; Kahn, M.L.
    Isolation and characterization of a novel glutamine synthetase from Rhizobium meliloti (1993), J. Biol. Chem., 268, 469-475.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP enzyme form GSIII Sinorhizobium meliloti
diphosphate enzyme form GSIII Sinorhizobium meliloti
L-methionine sulfoximine the concentration needed to inhibit GSIII is 50-100times higher than that needed to inhibit GSI or GSII Sinorhizobium meliloti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3
-
hydroxylamine enzyme form GSIII Sinorhizobium meliloti
13.3
-
Glu enzyme form GSIII Sinorhizobium meliloti
33
-
NH4+ enzyme form GSIII Sinorhizobium meliloti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46500
-
x * 46500 + x * 49000, SDS-PAGE Sinorhizobium meliloti
49000
-
x * 46500 + x * 49000, SDS-PAGE Sinorhizobium meliloti
355000
-
gel filtration Sinorhizobium meliloti

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti
-
3 enzyme forms: GSI, GSII and GSIII
-

Purification (Commentary)

Purification (Comment) Organism
GSIII Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
Sinorhizobium meliloti ADP + phosphate + L-Gln
-
?
ATP + L-glutamate + hydroxylamine
-
Sinorhizobium meliloti ADP + phosphate + gamma-glutamyl hydroxamate
-
?

Subunits

Subunits Comment Organism
octamer x * 46500 + x * 49000, SDS-PAGE Sinorhizobium meliloti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
enzyme form GSIII Sinorhizobium meliloti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
enzyme form GSIII Sinorhizobium meliloti