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Literature summary for 6.3.1.2 extracted from

  • Robertson, D.L.; Alberte, R.S.
    Isolation and characterization of glutamine synthetase from the marine diatom Skeletonema costatum (1996), Plant Physiol., 111, 1169-1175.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.1.2

General Stability

General Stability Organism
isoform GS1 is more labile than isoform GS2 in the early stages of purification Skeletonema costatum

Inhibitors

Inhibitors Comment Organism Structure
Ala
-
 Skeletonema costatum
Gly
-
 Skeletonema costatum
L-methionine sulfoximine
-
 Skeletonema costatum
Phosphinothricin
-
 Skeletonema costatum
Ser
-
 Skeletonema costatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 ATP
-
 Skeletonema costatum
0.7
-
 NH4+
-
 Skeletonema costatum
5.7
-
 Glu
-
 Skeletonema costatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 6 * 70000, SDS-PAGE Skeletonema costatum
400000
-
 isoform GS2, gel filtration Skeletonema costatum

Organism

Organism UniProt Comment Textmining
Skeletonema costatum
-
 isoform GS1 and GS2
-
Skeletonema costatum CCMP 1332
-
 isoform GS1 and GS2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Skeletonema costatum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.27
-
-
 Skeletonema costatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
 Skeletonema costatum ADP + phosphate + L-Gln
-
 ?
ATP + L-Glu + NH4+
-
 Skeletonema costatum CCMP 1332 ADP + phosphate + L-Gln
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 70000, SDS-PAGE Skeletonema costatum