Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ala | beta-Ala; inhibition of enzyme form GSII and GSIII | Rhizobium phaseoli | |
L-methionine sulfoximine | inhibition of enzyme form GSII and GSIII | Rhizobium phaseoli | |
L-methionine sulfoximine | - |
Sinorhizobium meliloti | |
Mg2+ | inhibits enzyme form GSIII to nearly 72%, enzyme form GSI 30%, and enzyme form GSII 33.3% | Rhizobium phaseoli | |
thymidine | complete inhibition of enzyme form GSII, partial inhibition of enzyme form GSI, no effect on enzyme form GSIII | Rhizobium phaseoli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64570 | - |
enzyme form GSI, gel filtration | Rhizobium phaseoli |
489800 | - |
enzyme form GSII, gel filtration | Rhizobium phaseoli |
891000 | - |
enzyme form GSIII, gel filtration | Rhizobium phaseoli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium leguminosarum | - |
3 enzyme forms: GSI, GSII and GSIII | - |
Rhizobium phaseoli | - |
- |
- |
Sinorhizobium meliloti | - |
3 enzyme forms: GSI, GSII and GSIII | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | - |
Sinorhizobium meliloti | ADP + phosphate + L-Gln | - |
? | |
ATP + L-Glu + NH4+ | - |
Rhizobium leguminosarum | ADP + phosphate + L-Gln | - |
? | |
ATP + L-Glu + NH4+ | - |
Rhizobium phaseoli | ADP + phosphate + L-Gln | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
20.5% inactivation of enzyme form GSI, 55.4% inactivation of enzyme form GSII, 27.3% inactivation of enzyme form GSIII | Rhizobium phaseoli |
60 | - |
15 min, 40% inactivation of enzyme form GSI, 74.6% inactivation of enzyme form GSII, 35.5% inactivation of enzyme form GSIII | Rhizobium leguminosarum |
60 | - |
15 min, 45.8% inactivation of enzyme form GSI, 61.2% inactivation of enzyme form GSII, 37.5% inactivation of enzyme form GSIII | Sinorhizobium meliloti |