Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | cathepsin F is secreted as an inactive zymogen that autocatalytically processes and activates to a mature enzyme at pH 4.5 (after 6 h incubation) via an intermolecular cleavage at the prosegment-mature domain junction, the active cathepsin B1 zymogen is capable of trans-activating cathepsin F by proteolytic removal of its prosegment at pH 5.5 | Opisthorchis viverrini |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
cathepsin F proenzyme in the yeast medium is present as two major protein bands migrating at 41000 Da and 47000 Da as a result of differential addition of N-linked glycans | Opisthorchis viverrini |
47000 | - |
cathepsin F proenzyme in the yeast medium is present as two major protein bands migrating at 41000 Da and 47000 Da as a result of differential addition of N-linked glycans | Opisthorchis viverrini |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hemoglobin + H2O | Opisthorchis viverrini | cathepsin F and cathepsin B can work in concert to hydrolyse hemoglobin at low pH | ? | - |
? | |
additional information | Opisthorchis viverrini | cathepsin F degrades extracellular matrix proteins more effectively than capthespin B1 at physiological pH | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Opisthorchis viverrini | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Opisthorchis viverrini |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adult | - |
Opisthorchis viverrini | - |
egg | - |
Opisthorchis viverrini | - |
metacercaria | - |
Opisthorchis viverrini | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | low activity | Opisthorchis viverrini | ? | - |
? | |
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O | high activity | Opisthorchis viverrini | benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | high activity | Opisthorchis viverrini | ? | - |
? | |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O | very low activity | Opisthorchis viverrini | ? | - |
? | |
Hemoglobin + H2O | cathepsin F and cathepsin B can work in concert to hydrolyse hemoglobin at low pH | Opisthorchis viverrini | ? | - |
? | |
additional information | cathepsin F degrades extracellular matrix proteins more effectively than capthespin B1 at physiological pH | Opisthorchis viverrini | ? | - |
? | |
additional information | the fully activated cathepsin F cleaves fluorogenic-NHMec substrates with the preference Val-Leu-Arg>Leu-Arg>Phe-Arg>Arg-Arg with little or no activity against Pro-Arg or Pro-Lys substrates | Opisthorchis viverrini | ? | - |
? | |
Pro-Arg-7-amido-4-methylcoumarin + H2O | very low activity | Opisthorchis viverrini | ? | - |
? | |
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O | very low activity | Opisthorchis viverrini | ? | - |
? | |
tert-butyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O | highest activity | Opisthorchis viverrini | ? | - |
? | |
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O | very low activity | Opisthorchis viverrini | ? | - |
? | |
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O | very low activity | Opisthorchis viverrini | ? | - |
? | |
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin + H2O | very low activity | Opisthorchis viverrini | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CF-1 | - |
Opisthorchis viverrini |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Opisthorchis viverrini |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8.5 | - |
Opisthorchis viverrini |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
cathepsin F zymogen is inactive at pH 5.5 | Opisthorchis viverrini |