Cloned (Comment) | Organism |
---|---|
- |
Vicia sativa |
expression of inactive precursor enzyme in yeast and insect cells, expression of a processing-site mutant in yeast and subsequent in vitro processing study | Ricinus communis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Vicia sativa | 5618 | - |
cell wall | - |
Arabidopsis thaliana | 5618 | - |
cell wall | - |
Ricinus communis | 5618 | - |
cell wall | - |
Nicotiana tabacum | 5618 | - |
ER body | special proteinase-storage vesicle that contain the enzyme precursor, which is probably packed together with its storage-protein substrates | Arabidopsis thaliana | 10168 | - |
additional information | proenzyme is probably attached to its storage-protein substrates | Ricinus communis | - |
- |
additional information | proenzyme is probably attached to its storage-protein substrates | Nicotiana tabacum | - |
- |
vacuole | protein storage vacuoles | Vicia sativa | 5773 | - |
vacuole | protein storage vacuoles | Arabidopsis thaliana | 5773 | - |
vacuole | protein storage vacuoles | Ricinus communis | 5773 | - |
vacuole | protein storage vacuoles | Nicotiana tabacum | 5773 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
11S proglobulin + H2O | Vicia sativa | processing to the active trimer | ? | - |
? | |
11S proglobulin + H2O | Arabidopsis thaliana | processing to the active trimer | ? | - |
? | |
11S proglobulin + H2O | Ricinus communis | processing to the active trimer | ? | - |
? | |
11S proglobulin + H2O | Canavalia ensiformis | processing to the active trimer | ? | - |
? | |
11S proglobulin + H2O | Nicotiana tabacum | processing to the active trimer | ? | - |
? | |
additional information | Vicia sativa | during germination and seedling growth legumains might also act as processing enzymes, protein processing and degradation by legumains, overview | ? | - |
? | |
additional information | Arabidopsis thaliana | during germination and seedling growth legumains might also act as processing enzymes, protein processing and degradation by legumains, overview | ? | - |
? | |
additional information | Ricinus communis | during germination and seedling growth legumains might also act as processing enzymes, protein processing and degradation by legumains, overview | ? | - |
? | |
additional information | Canavalia ensiformis | during germination and seedling growth legumains might also act as processing enzymes, protein processing and degradation by legumains, overview | ? | - |
? | |
additional information | Nicotiana tabacum | during germination and seedling growth legumains might also act as processing enzymes, protein processing and degradation by legumains, overview | ? | - |
? | |
papain-like cysteine proteinase precursors + H2O | Arabidopsis thaliana | involved in the senescing of tissues and cell death | activated papin-like cysteine proteinases | - |
? | |
precursor of basic vacuolar chitinase + H2O | Arabidopsis thaliana | involved in the senescing of tissues and cell death | active basic vacuolar chitinase | - |
? | |
protein + H2O | Ricinus communis | - |
peptides | - |
? | |
protein + H2O | Canavalia ensiformis | - |
peptides | - |
? | |
protein + H2O | Nicotiana tabacum | - |
peptides | - |
? | |
protein + H2O | Vicia sativa | enzyme is involved mainly in storage protein breakdown | peptides | - |
? | |
protein + H2O | Arabidopsis thaliana | involved in mobilization of storage proteins and stress response processes | peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
4 genes, 3 of them encoded in a cluster, 2 isoforms of vegetative vacuolar processing enzymes alpha-VPE and beta-VPE | - |
Canavalia ensiformis | - |
Jack bean | - |
Nicotiana tabacum | Q9XG75 | - |
- |
Ricinus communis | - |
- |
- |
Vicia sativa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | autocatalytic processing of inactive proenzyme to the active mature enzyme by removal of C- and N-terminally propeptides, activation takes place in the vacuole or cell wall | Nicotiana tabacum |
proteolytic modification | autocatalytic processing of inactive proenzyme to the active mature enzyme by removal of C- and N-terminally propeptides, activation takes place in the vacuole or cell wall after fusion with the ER bodies | Vicia sativa |
proteolytic modification | autocatalytic processing of inactive proenzyme to the active mature enzyme by removal of C- and N-terminally propeptides, activation takes place in the vacuole or cell wall after fusion with the ER bodies | Arabidopsis thaliana |
proteolytic modification | autocatalytic processing of inactive proenzyme to the active mature enzyme by removal of C- and N-terminally propeptides, activation takes place in the vacuole or cell wall, precursor is cleaved at sites containing Asn residues at the P1 position | Ricinus communis |
Purification (Comment) | Organism |
---|---|
from cotyledon | Vicia sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Vicia sativa | - |
embryo | - |
Nicotiana tabacum | - |
epidermal cell | attached to storage-protein substrates in ER bodies | Arabidopsis thaliana | - |
leaf | wounded or senescing, alpha-VPE and beta-VPE | Arabidopsis thaliana | - |
additional information | in all vegetative organs, the precursor enzyme is synthesized at membrane-bound polysomes in the cytoplasm | Nicotiana tabacum | - |
additional information | the precursor enzyme is synthesized at membrane-bound polysomes in the cytoplasm | Vicia sativa | - |
additional information | the precursor enzyme is synthesized at membrane-bound polysomes in the cytoplasm | Ricinus communis | - |
additional information | VPE isoforms are also found during stress response of plant organ, e.g. wounded leaves, the precursor enzyme is synthesized at membrane-bound polysomes in the cytoplasm | Arabidopsis thaliana | - |
root | undergoing controlled cell death, alpha-VPE | Arabidopsis thaliana | - |
seed | - |
Vicia sativa | - |
seed | - |
Arabidopsis thaliana | - |
seed | - |
Ricinus communis | - |
seed | - |
Nicotiana tabacum | - |
seed | maturing | Canavalia ensiformis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11S proglobulin + H2O | degradation of unassembled proteins or misfolded precursors | Vicia sativa | ? | - |
? | |
11S proglobulin + H2O | degradation of unassembled proteins or misfolded precursors | Arabidopsis thaliana | ? | - |
? | |
11S proglobulin + H2O | degradation of unassembled proteins or misfolded precursors | Ricinus communis | ? | - |
? | |
11S proglobulin + H2O | degradation of unassembled proteins or misfolded precursors | Canavalia ensiformis | ? | - |
? | |
11S proglobulin + H2O | degradation of unassembled proteins or misfolded precursors | Nicotiana tabacum | ? | - |
? | |
11S proglobulin + H2O | processing to the active trimer | Vicia sativa | ? | - |
? | |
11S proglobulin + H2O | processing to the active trimer | Arabidopsis thaliana | ? | - |
? | |
11S proglobulin + H2O | processing to the active trimer | Ricinus communis | ? | - |
? | |
11S proglobulin + H2O | processing to the active trimer | Canavalia ensiformis | ? | - |
? | |
11S proglobulin + H2O | processing to the active trimer | Nicotiana tabacum | ? | - |
? | |
concanavalin A precursor + H2O | internal excision of the propeptide and transpeptidation that leads to the inverted fusion of the 2 fragments of the precursor | Canavalia ensiformis | activated concanavalin A | - |
? | |
additional information | during germination and seedling growth legumains might also act as processing enzymes, protein processing and degradation by legumains, overview | Vicia sativa | ? | - |
? | |
additional information | during germination and seedling growth legumains might also act as processing enzymes, protein processing and degradation by legumains, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | during germination and seedling growth legumains might also act as processing enzymes, protein processing and degradation by legumains, overview | Ricinus communis | ? | - |
? | |
additional information | during germination and seedling growth legumains might also act as processing enzymes, protein processing and degradation by legumains, overview | Canavalia ensiformis | ? | - |
? | |
additional information | during germination and seedling growth legumains might also act as processing enzymes, protein processing and degradation by legumains, overview | Nicotiana tabacum | ? | - |
? | |
octapeptides basing on the processing sites of 11S proglobulin + H2O | - |
Vicia sativa | ? | - |
? | |
papain-like cysteine proteinase precursors + H2O | cleavage of C-terminal propeptide | Arabidopsis thaliana | activated papin-like cysteine proteinases | - |
? | |
papain-like cysteine proteinase precursors + H2O | involved in the senescing of tissues and cell death | Arabidopsis thaliana | activated papin-like cysteine proteinases | - |
? | |
precursor of basic vacuolar chitinase + H2O | cleavage of C-terminal propeptide | Arabidopsis thaliana | active basic vacuolar chitinase | - |
? | |
precursor of basic vacuolar chitinase + H2O | involved in the senescing of tissues and cell death | Arabidopsis thaliana | active basic vacuolar chitinase | - |
? | |
protein + H2O | - |
Ricinus communis | peptides | - |
? | |
protein + H2O | - |
Canavalia ensiformis | peptides | - |
? | |
protein + H2O | - |
Nicotiana tabacum | peptides | - |
? | |
protein + H2O | enzyme is involved mainly in storage protein breakdown | Vicia sativa | peptides | - |
? | |
protein + H2O | involved in mobilization of storage proteins and stress response processes | Arabidopsis thaliana | peptides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
As-specific vacuolar cysteine proteinase | - |
Vicia sativa |
Asn-specific vacuolar cysteine proteinase | - |
Arabidopsis thaliana |
Asn-specific vacuolar cysteine proteinase | - |
Ricinus communis |
Asn-specific vacuolar cysteine proteinase | - |
Canavalia ensiformis |
Asn-specific vacuolar cysteine proteinase | - |
Nicotiana tabacum |