Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM | Drosophila melanogaster | |
Co2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM | Drosophila melanogaster | |
Cu2+ | 56% inhibition at 0.1 mM | Drosophila melanogaster | |
EDTA | 80% inhibition at 0.1 mM | Drosophila melanogaster | |
Mg2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM | Drosophila melanogaster | |
Mn2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM with substrates substance P and bradykinin | Drosophila melanogaster | |
Ni2+ | complete inhibition at 0.001 mM | Drosophila melanogaster | |
Zn2+ | complete inhibition at 0.001 mM | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Drosophila melanogaster | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM | Drosophila melanogaster | |
Co2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM | Drosophila melanogaster | |
Mg2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM | Drosophila melanogaster | |
Mn2+ | activates the enzyme at concentrations of 0.01-0.1 mM with substrate substance P, inhibits above 1 mM with substrates substance P and bradykinin | Drosophila melanogaster | |
additional information | metalloprotease | Drosophila melanogaster |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
x * 71000, recombinant His-tagged enzyme, SDS-PAGE | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | O96794 | cytosolic isoform | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | adult fly, high level | Drosophila melanogaster | - |
gut | adult fly | Drosophila melanogaster | - |
additional information | developmentally regulated | Drosophila melanogaster | - |
ovary | adult fly | Drosophila melanogaster | - |
testis | adult fly | Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bradykinin + H2O | i.e. RPPGFSPFR, hydrolysis of the N-terminal Arg1-Pro2 bond | Drosophila melanogaster | Arg + des-Arg-bradykinin | i.e. PPGFSPFR | ? | |
substance P + H2O | i.e. RPKPQQFFGLM, hydrolysis of the N-terminal Arg1-Pro2 bond | Drosophila melanogaster | Arg + des-Arg-substance P | i.e. PKPQQFFGLM | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 71000, recombinant His-tagged enzyme, SDS-PAGE | Drosophila melanogaster |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase P | - |
Drosophila melanogaster |
DAP-P | - |
Drosophila melanogaster |
More | the enzyme belongs to the peptidase family M24 | Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
with substrate substance P | Drosophila melanogaster |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8 | - |
Drosophila melanogaster |