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Literature summary for 3.2.1.24 extracted from

  • Tulsiani, D.R.P.; Coleman, V.D.; Touster, O.
    Rat epididymal alpha-D-mannosidase: purification, carbohydrate composition, substrate specificity, and antibody production (1988), Arch. Biochem. Biophys., 267, 60-68.
    View publication on PubMed
Search for more literature for 3.2.1.24

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10.7
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O Rattus sp.
-
 alpha-D-mannopyranose + ?
-
 ?
Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O Rattus sp.
-
 alpha-D-mannopyranose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains mainly N-linked oligosaccharides, small amounts of fucose, galactose, glucose Rattus sp.

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
epididymis
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
153
-
-
 Rattus sp.

Storage Stability

Storage Stability Organism
0-4°C, pH 6.0, several weeks Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
-
 Rattus sp. alpha-D-mannopyranose + ?
-
 ?
Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
-
 Rattus sp. alpha-D-mannopyranose + ?
-
 ?
p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Rattus sp. p-nitrophenol + alpha-D-mannopyranose
-
 ?

Subunits

Subunits Comment Organism
oligomer composed of 60 and 31 kDa subunits Rattus sp.

Synonyms

Synonyms Comment Organism
lysosomal alpha-D-mannosidase
-
 Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4 4.6
-
 Rattus sp.