Cloned (Comment) | Organism |
---|---|
gene Bmcce-5, DNA and amino acid sequence determination and analysis, intron-exon structures, sequence comparisons and phylogenetic analysis, recombinant expression of BmCCE-5 as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus system | Bombyx mori |
gene Bmjhe, DNA and amino acid sequence determination and analysis, intron-exon structures, sequence comparisons and phylogenetic analysis of JHE, recombinant expression of BmJHE as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus system | Bombyx mori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
juvenile hormone III | pH 7.4, 22°C, recombinant BmCCE-5 | Bombyx mori | |
0.0028 | - |
juvenile hormone III | pH 7.4, 22°C, recombinant BmJHE | Bombyx mori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
juvenile hormone III + H2O | Bombyx mori | BmJHE is a juvenile hormone-selective esterase. Of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | - |
gene Bmjhe | - |
Bombyx mori | D2KWC2 | gene Bmcce-5 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gut | strong expression of Bmcce-5 | Bombyx mori | - |
malpighian tubule | strong expression of Bmcce-5 | Bombyx mori | - |
additional information | developmental expression patterns of gene Bmcce-5 by real-time quantitative PCR analysis, the expression pattern varies among developmental stages and organs | Bombyx mori | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
juvenile hormone III + H2O | - |
Bombyx mori | ? | - |
? | |
juvenile hormone III + H2O | BmJHE is a juvenile hormone-selective esterase. Of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm | Bombyx mori | ? | - |
? | |
juvenile hormone III + H2O | BmCCE-5 has the ability to degrade juvenile hormone, however, the protein might not function as a JH-specific esterase in vivo as it has a high Km value for juvenile hormone | Bombyx mori | ? | - |
? | |
methyl heptylthioacetothioate + H2O | a synthetic substrate | Bombyx mori | ? | - |
? | |
additional information | the enzymes encoded by genes Bmcce-1-4 are not active on juvenile hormone | Bombyx mori | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BmCCE-5 | - |
Bombyx mori |
JH-specific esterase | - |
Bombyx mori |
JHE | - |
Bombyx mori |
juvenile hormone-selective esterase | - |
Bombyx mori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Bombyx mori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | 0.018 | juvenile hormone III | pH 7.4, 22°C, recombinant BmCCE-5 | Bombyx mori | |
1 | - |
juvenile hormone III | pH 7.4, 22°C, recombinant BmJHE | Bombyx mori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bombyx mori |
General Information | Comment | Organism |
---|---|---|
physiological function | Bmcce-5 is strongly expressed in Malpighian tubules and the gut, it might function in digestion or xenobiotic metabolism | Bombyx mori |
physiological function | BmJHE, a juvenile hormone-selective esterase, JHE, is important for juvenile hormone titer regulation in the silkworm Bombyx mori | Bombyx mori |