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Literature summary for 2.7.7.43 extracted from

  • Abeln, M.; Borst, K.M.; Cajic, S.; Thiesler, H.; Kats, E.; Albers, I.; Kuhn, M.; Kaever, V.; Rapp, E.; Muenster-Kuehnel, A.; Weinhold, B.
    Sialylation is dispensable for early murine embryonic development in vitro (2017), ChemBioChem, 18, 1305-1316 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.43

Cloned(Commentary)

Cloned (Comment) Organism
gene Cmas, quantitative PCR expression analysis Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information generation of mouse embryonic stem cell (mESC) lines that lack CMP-Sia synthetase (CMAS) and thereby the ability to activate Sia to CMP-Sia, phenotype, overview. The Cmas targeting strategy uses a targeting vector with diphtheria toxin cassette (DT) to increase homologous recombination. Cmas-/- mESC accumulate intracellular Neu5Ac. alpha2,3- and alpha2,6-Sialylated N-glycans are absent in Cmas-/- mESCs Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + N-acylneuraminate Mus musculus
-
 diphosphate + CMP-N-acylneuraminate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q99KK2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryonic stem cell
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + N-acylneuraminate
-
 Mus musculus diphosphate + CMP-N-acylneuraminate
-
 ?

Synonyms

Synonyms Comment Organism
CMAS
-
 Mus musculus
CMP-Sia synthetase
-
 Mus musculus

General Information

General Information Comment Organism
malfunction mouse embryonic stem cell (mESC) lines that lack CMP-Sia synthetase (CMAS) and thereby the ability to activate Sia to CMP-Sia show that loss of CMAS activity results in an asialo cell surface accompanied by an increase in glycoconjugates with terminal galactosyl and oligo-LacNAc residues, as well as intracellular accumulation of free Sia. These changes do not impact intracellular metabolites or the morphology and transcriptome of pluripotent mESC lines. Moreover, the capacity of Cmas-/- mESCs for undirected differentiation into embryoid bodies, germ layer formation and even the generation of beating cardiomyocytes provides first and conclusive evidence that pluripotency and differentiation of mESC in vitro can proceed in the absence of (poly)sialoglycans. Genetic ablation of CMAS results in complete loss of cellsurface sialylation with concomitant increase in LacNAc structures. Intracellular Neu5Ac accumulation alters neither associated metabolites nor intracellular glycosylation Mus musculus
metabolism the enzyme is involved in the biosynthesis of sialoglycoconjugates in vertebrates, overview Mus musculus
physiological function CMAS activity is essential for maintaining cellular homeostasis of sialic acid biosynthesis Mus musculus