Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | - |
Homo sapiens |
Application | Comment | Organism |
---|---|---|
pharmacology | the enzyme is a target for development of anticancer drugs | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
overexpression of the enzyme in glioma T98-G cells causes a 10fold increased resistance to inhibitor NH125 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | non-specific inhibitors of the enzyme cause cell death | Homo sapiens | |
NH125 | an imidazolium histidine kinase inhibitor also inhibits the eukaryotic eEF-2 kinase enzyme in vitro and in vivo, IC50 is 60 nM, decreases viability of cancer cell lines with IC50S of 0.0007 to 0.0048 mM, overview | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens | |
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [elongation factor 2] | Homo sapiens | - |
ADP + [elongation factor 2]phosphate | - |
? | |
additional information | Homo sapiens | the enzyme expression and activity is increased in several forms of malignancy and human cancer, non-specific inhibitors of the enzyme cause cell death | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-172 cell | glioblastoma cell line | Homo sapiens | - |
A-2780 cell | ovarian carcinoma cell line | Homo sapiens | - |
HeLa cell | cervical carcinoma cell line | Homo sapiens | - |
MCF-7 cell | breast carcinoma cell line | Homo sapiens | - |
additional information | the enzyme expression and activity is increased in several forms of malignancy | Homo sapiens | - |
OVCAR-3 cell | ovarian carcinoma cell line | Homo sapiens | - |
PC-3 cell | prostate carcinoma cell line | Homo sapiens | - |
T-98G cell | glioblastoma cell line | Homo sapiens | - |
U-138MG cell | glioblastoma cell line | Homo sapiens | - |
U-87MG cell | glioblastoma cell line | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [elongation factor 2] | - |
Homo sapiens | ADP + [elongation factor 2]phosphate | - |
? | |
additional information | the enzyme expression and activity is increased in several forms of malignancy and human cancer, non-specific inhibitors of the enzyme cause cell death | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
eEF-2 kinase | - |
Homo sapiens |
eukaryotic elongation factor 2 kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00006 | - |
an imidazolium histidine kinase inhibitor also inhibits the eukaryotic eEF-2 kinase enzyme in vitro and in vivo, IC50 is 60 nM | Homo sapiens | NH125 | |
0.0007 | 0.0048 | decreases viability of cancer cell lines with IC50S of 0.0007 to 0.0048 mM, overview | Homo sapiens | NH125 |