Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Runella slithyformis |
Protein Variants | Comment | Organism |
---|---|---|
D254A | inactive | Runella slithyformis |
H73A | the mutant shows wild type activity | Runella slithyformis |
R257A | inactive | Runella slithyformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.104 | - |
ATP | at pH 8.0 and 37°C | Runella slithyformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on Mg2+, 5 mM used in assay conditions | Runella slithyformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | Runella slithyformis | - |
ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | Runella slithyformis ATCC 49304 | - |
ADP + 5'-phospho-DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Runella slithyformis | - |
- |
- |
Runella slithyformis ATCC 49304 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His60 Ni Superflow resin column chromatography | Runella slithyformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-CTAGAGCTACAATTGCGACCG-3' | - |
Runella slithyformis | ADP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3' | - |
? | |
ATP + 5'-CTAGAGCTACAATTGCGACCG-3' | - |
Runella slithyformis ATCC 49304 | ADP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3' | - |
? | |
ATP + 5'-dephospho-DNA | - |
Runella slithyformis | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | - |
Runella slithyformis ATCC 49304 | ADP + 5'-phospho-DNA | - |
? | |
CTP + 5'-CTAGAGCTACAATTGCGACCG-3' | - |
Runella slithyformis | CDP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3' | - |
? | |
CTP + 5'-CTAGAGCTACAATTGCGACCG-3' | - |
Runella slithyformis ATCC 49304 | CDP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3' | - |
? | |
dATP + 5'-CTAGAGCTACAATTGCGACCG-3' | - |
Runella slithyformis | dADP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3' | - |
? | |
dATP + 5'-CTAGAGCTACAATTGCGACCG-3' | - |
Runella slithyformis ATCC 49304 | dADP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3' | - |
? | |
GTP + 5'-CTAGAGCTACAATTGCGACCG-3' | - |
Runella slithyformis | GDP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3' | - |
? | |
additional information | the enzyme also has RNA 2',3-cyclic phosphodiesterase, DNA 3'-phosphatase and RNA 2'- and 3'-phosphomonoesterase activities | Runella slithyformis | ? | - |
- |
|
additional information | the enzyme also has RNA 2',3-cyclic phosphodiesterase, DNA 3'-phosphatase and RNA 2'- and 3'-phosphomonoesterase activities | Runella slithyformis ATCC 49304 | ? | - |
- |
|
UTP + 5'-CTAGAGCTACAATTGCGACCG-3' | - |
Runella slithyformis | UDP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3' | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE | Runella slithyformis |
Synonyms | Comment | Organism |
---|---|---|
5'-OH polynucleotide kinase | - |
Runella slithyformis |
HD-Pnk | the enzyme consists of an N-terminal 2',3'-phosphoesterase module of the HD domain family fused to a C-terminal polynucleotide kinase module | Runella slithyformis |
polynucleotide kinase | - |
Runella slithyformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.183 | - |
ATP | at pH 6.0 and 37°C | Runella slithyformis |