Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | - |
Rattus norvegicus | |
p-chloromercuribenzoate | reversible by 2-mercaptoethanol | Rattus norvegicus | |
phosphate | weak | Rattus norvegicus | |
sulfate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Rattus norvegicus | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Rattus norvegicus | |
Ca2+ | Mn2+ or Ca2+ effective in activation | Rattus norvegicus | |
Co2+ | less effective | Rattus norvegicus | |
divalent cations | absolutely required | Rattus norvegicus | |
Mg2+ | - |
Rattus norvegicus | |
Mn2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
Mn2+ | Mn2+ or Ca2+ effective | Rattus norvegicus | |
Zn2+ | less effective | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | Rattus norvegicus | - |
ADP + 5'-phospho-DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
261fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0418 | - |
purified enzyme | Rattus norvegicus |
Storage Stability | Organism |
---|---|
0-4°C, stable for at least 1 week | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | - |
Rattus norvegicus | ADP + 5'-phospho-DNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | about 80-86% of maximal activity at pH 7.0 and pH 4.5 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
diphosphate | pH 5.5, 37°C | Rattus norvegicus | |
0.5 | - |
sulfate | pH 5.5, 37°C | Rattus norvegicus |