Cloned (Comment) | Organism |
---|---|
gene gsk, several metabolic enzymes such as GMP synthetase and IMP dehydrogenase, GMP reductase, and guanosine-inosine kinase, all involved in the biosynthesis of GMP, are co-overexpressed in recombinant Escherichia coli TOP10 and BL21star(DE3) cells becoming able to produce GDP-L-fucose, pH-stat fed-batch fermentations quantification and optimization, overview | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of Gsk in the fed-batch fermentation in recombinant Escherichia coli strain BL21star(DE3) with glucose as the sole carbon source, leads to a significant improvement of GDP-L-fucose production, profiles of cell growth and GDP-L-fucose production, overview | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + guanosine | Escherichia coli | - |
ADP + GMP | - |
? | |
ATP + inosine | Escherichia coli | - |
ADP + IMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
ATCC 10798, gene gsk | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + guanosine | - |
Escherichia coli | ADP + GMP | - |
? | |
ATP + inosine | - |
Escherichia coli | ADP + IMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Gsk | - |
Escherichia coli |
guanosine-inosine kinase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | in the salvage pathway, guanosine and inosine are directly phosphorylated to GMP and IMP, respectively, by guanosine-inosine kinase, or guanosine is indirectly converted to GMP through serial phosphorolysis and phosphorylation catalyzed by purine nucleoside phosphorylase and guanine phosphoribosyltransferase, respectively, pathway, overview | Escherichia coli |