Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine | 35% inhibition at 1 mM, 20% inhibition at 4 mM and above, enzyme form QI and QII | Spinacia oleracea | |
Wortmannin | IC50 for QI is approximately 0.007 mM, enzyme form QII is inhibited 30% at 0.01 mM | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
phosphatidylinositol | pH 7.5, enzyme form QII | Spinacia oleracea | |
0.23 | - |
phosphatidylinositol | pH 7.5, enzyme form QI | Spinacia oleracea | |
0.44 | - |
ATP | pH 7.5, enzyme form QII | Spinacia oleracea | |
0.49 | - |
ATP | pH 7.5, enzyme form QI | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | firmly associated with plasma membrane | Spinacia oleracea | 16020 | - |
plasma membrane | formyl associated with plasma membrane | Spinacia oleracea | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibits enzyme form QI and QII at millimolar concentrations | Spinacia oleracea | |
Mg2+ | Mg2+ is the preferred divalent cation | Spinacia oleracea | |
Mn2+ | Mn2+ can partially substitute for Mg2+ in the reaction catalyzed by the QII enzyme but not in that catalyzed by QI. Mn2+ acts synergistically, with suboptimal Mg2+ concentrations to activate not only the QII enzyme, but also to some extent QI | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, SDS-PAGE | Spinacia oleracea |
120000 | - |
x * 120000, SDS-PAGE | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, enzyme form QI and QII | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol | - |
Spinacia oleracea | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Spinacia oleracea |
monomer | x * 120000, SDS-PAGE | Spinacia oleracea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | pH 7.0: about 50% of maximal activity, pH 8.5: about 40% of maximal activity, enzyme form QII | Spinacia oleracea |
7 | 8 | pH 7.0: about 70% of maximal activity, pH 8.0: about 45% of maximal activity | Spinacia oleracea |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.007 | - |
IC50 for QI is approximately 0.007 mM, enzyme form QII is inhibited 30% at 0.01 mM | Spinacia oleracea | Wortmannin |