Cloned (Comment) | Organism |
---|---|
gene coaE, DNA sequence determination and analysis, overexpression in Escherichia coli strain W3110 | Corynebacterium ammoniagenes |
gene yacE, DNA sequence analysis | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
ATP | native wild-type enzyme, pH 8.5, 25°C | Corynebacterium ammoniagenes | |
0.76 | - |
dephospho-CoA | native wild-type enzyme, pH 8.5, 25°C | Corynebacterium ammoniagenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22200 | - |
recombinant enzyme, gel filtration | Corynebacterium ammoniagenes |
22600 | - |
1 * 22600, deduced from DNA sequence | Escherichia coli |
25000 | - |
native enzyme, gel filtration | Corynebacterium ammoniagenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dephospho-CoA | Corynebacterium ammoniagenes | last step of coenzyme A biosynthesis: phosphorylation of the 3'-OH group of the ribose sugar moiety | ADP + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium ammoniagenes | - |
gene coaE | - |
Escherichia coli | P0A6I9 | gene caoE, formerly yacE | - |
Purification (Comment) | Organism |
---|---|
native wild-type enzyme, 2800fold to homogeneity | Corynebacterium ammoniagenes |
recombinant wild-type from Escherichia coli W3110, 22fold | Corynebacterium ammoniagenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.5 | - |
purified native wild-type enzyme | Corynebacterium ammoniagenes |
22 | - |
purified recombinant wild-type enzyme | Corynebacterium ammoniagenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dephospho-CoA | - |
Corynebacterium ammoniagenes | ADP + CoA | - |
ir | |
ATP + dephospho-CoA | - |
Escherichia coli | ADP + CoA | - |
ir | |
ATP + dephospho-CoA | last step of coenzyme A biosynthesis: phosphorylation of the 3'-OH group of the ribose sugar moiety | Corynebacterium ammoniagenes | ADP + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 1 * 22600, deduced from DNA sequence | Escherichia coli |
monomer | 1 * 22200, recombinant wild-type enzyme, SDS-PAGE | Corynebacterium ammoniagenes |
monomer | 1 * 25000, native wild-type enzyme, SDS-PAGE | Corynebacterium ammoniagenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Corynebacterium ammoniagenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Escherichia coli |
8.5 | - |
recombinant enzyme, broad | Corynebacterium ammoniagenes |