Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-AI cells | Anopheles gambiae |
Crystallization (Comment) | Organism |
---|---|
in complex with with P1,P4-di(adenosine-5') tetraphosphate, sitting drop vapor diffusion method, using 25% (w/v) PEG3350, 0.2 M MgCl2, 5% (v/v) 2-propanol, 25% (v/v) glycerol and 0.1 M BisTris buffer pH 5.5, at 18°C | Anopheles gambiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodotubercidin | - |
Anopheles gambiae | |
additional information | 9-beta-D-arabinofuranosyladenine analogue of adenosine at concentrations in 150fold excess over adenosine does not affect the enzyme activity | Anopheles gambiae | |
P1,P3-di(adenosine-5')triphosphate | - |
Anopheles gambiae | |
P1,P4-di(adenosine-5')tetraphosphate | - |
Anopheles gambiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000081 | - |
adenosine | with ATP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.00023 | - |
adenosine | with GTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.00063 | - |
adenosine | with CTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.00074 | - |
adenosine | with UTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.00079 | - |
adenosine | with TTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.0014 | - |
ATP | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.049 | - |
CTP | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.054 | - |
GTP | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.056 | - |
UTP | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.245 | - |
TTP | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anopheles gambiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, gel filtration | Anopheles gambiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adult | - |
Anopheles gambiae | - |
Storage Stability | Organism |
---|---|
-80°C, purified enzyme in 10% (v/v) glycerol, several months, no loss of activity | Anopheles gambiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine | the enzyme is highly specific for adenosine and shows best activity with ATP as cosubstrate | Anopheles gambiae | ADP + AMP | - |
? | |
CTP + adenosine | - |
Anopheles gambiae | CDP + AMP | - |
? | |
GTP + adenosine | - |
Anopheles gambiae | GDP + AMP | - |
? | |
TTP + adenosine | - |
Anopheles gambiae | TDP + AMP | - |
? | |
UTP + adenosine | - |
Anopheles gambiae | UDP + AMP | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
adenosine | with CTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.035 | - |
adenosine | with UTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.056 | - |
adenosine | with TTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.061 | - |
UTP | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.062 | - |
CTP | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.16 | - |
adenosine | with ATP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.3 | - |
TTP | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.33 | - |
ATP | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.63 | - |
adenosine | with GTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
1.39 | - |
GTP | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000001 | - |
iodotubercidin | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.00086 | - |
P1,P4-di(adenosine-5')tetraphosphate | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
0.061 | - |
P1,P3-di(adenosine-5')triphosphate | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.09 | - |
UTP | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
1.2 | - |
TTP | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
1.27 | - |
CTP | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
26 | - |
GTP | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
240 | - |
ATP | at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
2700 | - |
adenosine | with GTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
4600 | - |
adenosine | with CTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
4700 | - |
adenosine | with UTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
7100 | - |
adenosine | with TTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae | |
19700 | - |
adenosine | with ATP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol | Anopheles gambiae |