Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
G212V | inhibition by phosphoenolpyruvate can not be reversed by GDP or ADP, neither ADP nor GDP bind well to the effector site | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MgATP2- | - |
Geobacillus stearothermophilus | |
additional information | product inhibition of reverse reaction | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Geobacillus stearothermophilus | |
0.028 | - |
D-fructose 6-phosphate | pH 8.2, 30°C, cosubstrate GTP | Geobacillus stearothermophilus | |
0.03 | - |
D-fructose 6-phosphate | pH 8.2, 30°C, at fructose 6-phosphate concentrations that are less than approx. one-third the fixed MgATP concentration | Geobacillus stearothermophilus | |
0.068 | - |
D-fructose 6-phosphate | pH 8.2, 30°C, cosubstrate UTP | Geobacillus stearothermophilus | |
0.07 | - |
ATP | pH 8.2, 30°C | Geobacillus stearothermophilus | |
0.15 | - |
D-fructose 6-phosphate | pH 8.2, 30°C, cosubstrate CTP | Geobacillus stearothermophilus | |
0.18 | - |
GTP | pH 8.2, 30°C | Geobacillus stearothermophilus | |
2.6 | - |
CTP | pH 8.2, 30°C | Geobacillus stearothermophilus | |
2.8 | - |
UTP | pH 8.2, 30°C | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
expressed in PFK-deficient Escherichia coli DF1020 cells | - |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + beta-D-fructofuranose 6-phosphate = ADP + beta-D-fructofuranose 1,6-bisphosphate | mechanism | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
160 | - |
- |
Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
-20°C, in 100 mM Tris-HCl buffer, pH 7.4, 1 mM dithiothreitol, 50% glycerol, stable | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | 35fold lower reverse reaction velocity | Geobacillus stearothermophilus | ADP + D-fructose 1,6-bisphosphate | - |
r | |
CTP + D-fructose 6-phosphate | - |
Geobacillus stearothermophilus | CDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | - |
Geobacillus stearothermophilus | GDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 6-phosphate | - |
Geobacillus stearothermophilus | UDP + D-fructose 1,6-bisphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
fructose 1,6-bisphosphate | reverse raction, recombinant enzyme | Geobacillus stearothermophilus | |
112 | - |
fructose 6-phosphate | forward reaction, recombinant enzyme | Geobacillus stearothermophilus |