General Stability | Organism |
---|---|
unstable in crude homogenate, iodoacetate, antipain, chymostatin or leupeptin stabilizes, not pepstatin, PMSF or soybean trypsin inhibitor | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dihydroxyacetone phosphate | weak | Spinacia oleracea | |
diphosphate | - |
Spinacia oleracea | |
glycerate 2-phosphate | - |
Spinacia oleracea | |
glycolate 2-phosphate | - |
Spinacia oleracea | |
additional information | phosphorylation by cAMP-dependent protein kinase, but no inhibition | Ricinus communis | |
additional information | - |
Spinacia oleracea | |
phosphoenolpyruvate | - |
Spinacia oleracea | |
sn-glycerol 3-phosphate | - |
Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data of native and phosphorylated enzyme | Spinacia oleracea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Spinacia oleracea | |
phosphate | activation | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
4 * 90000, H-form, SDS-PAGE | Spinacia oleracea |
90000 | - |
4 * 90000, H-form, SDS-PAGE | Ricinus communis |
90000 | - |
4 * 90000, H-form, SDS-PAGE | Helianthus tuberosus |
132000 | - |
L-form, gel filtration | Spinacia oleracea |
390000 | - |
H-form, gel filtration | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helianthus tuberosus | - |
Jerusalem artichoke | - |
Ricinus communis | - |
castor bean | - |
Spinacia oleracea | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Spinacia oleracea |
phosphoprotein | phosphorylation by cAMP-dependent protein kinase, but no inhibition | Ricinus communis |
Purification (Comment) | Organism |
---|---|
multiple molecular forms, predominant: H-(heavy) and L-(light)form | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Ricinus communis | - |
leaf | - |
Spinacia oleracea | - |
tuber | - |
Helianthus tuberosus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.718 | - |
- |
Spinacia oleracea |
Storage Stability | Organism |
---|---|
0°C, in 100 mM potassium acetate, 5 mM magnesium acetate, 2.5 mM DTT, 1 mM iodoacetate, 1 mg/ml antipain, 50 mM Tris-acetate, pH 7.8, several h | Spinacia oleracea |
0°C, L-form, at least 2 h in the absence of protease inhibitor | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Spinacia oleracea | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
ATP + beta-D-fructose 6-phosphate | - |
Ricinus communis | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
ATP + beta-D-fructose 6-phosphate | - |
Helianthus tuberosus | ADP + beta-D-fructose 2,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 90000, H-form, SDS-PAGE | Spinacia oleracea |
tetramer | 4 * 90000, H-form, SDS-PAGE | Ricinus communis |
tetramer | 4 * 90000, H-form, SDS-PAGE | Helianthus tuberosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Spinacia oleracea |
30 | - |
assay at | Ricinus communis |
30 | - |
assay at | Helianthus tuberosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Spinacia oleracea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
glycerate 2-phosphate | L form, pH 7.8, 30°C | Spinacia oleracea | |
0.018 | - |
phosphoenolpyruvate | L form, pH 7.8, 30°C | Spinacia oleracea | |
0.018 | - |
sn-glycerol 3-phosphate | L form, pH 7.8, 30°C | Spinacia oleracea | |
0.02 | - |
glycolate 2-phosphate | L form, pH 7.8, 30°C | Spinacia oleracea | |
0.026 | - |
diphosphate | L form, pH 7.8, 30°C | Spinacia oleracea | |
0.14 | - |
glycerate 2-phosphate | H form, pH 7.8, 30°C | Spinacia oleracea | |
0.15 | - |
phosphoenolpyruvate | H form, pH 7.8, 30°C | Spinacia oleracea | |
0.16 | - |
diphosphate | H form, pH 7.8, 30°C | Spinacia oleracea | |
0.16 | - |
sn-glycerol 3-phosphate | H form, pH 7.8, 30°C | Spinacia oleracea | |
0.18 | - |
glycolate 2-phosphate | H form, pH 7.8, 30°C | Spinacia oleracea | |
0.25 | - |
dihydroxyacetone phosphate | L form, pH 7.8, 30°C | Spinacia oleracea | |
1.9 | - |
dihydroxyacetone phosphate | H form, pH 7.8, 30°C | Spinacia oleracea |