Cloned (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
pyruvate | pH 8.0, 25°C | Thermus thermophilus | |
0.13 | - |
phenylpyruvate | pH 8.0, 25°C | Thermus thermophilus | |
0.14 | - |
4-hydroxyphenylpyruvate | pH 8.0, 25°C | Thermus thermophilus | |
0.16 | - |
Indolepyruvate | pH 8.0, 25°C | Thermus thermophilus | |
0.76 | - |
L-phenylalanine | pH 8.0, 25°C | Thermus thermophilus | |
0.82 | - |
L-tyrosine | pH 8.0, 25°C | Thermus thermophilus | |
1 | - |
L-asparagine | pH 8.0, 25°C | Thermus thermophilus | |
3.2 | - |
L-methionine | pH 8.0, 25°C | Thermus thermophilus | |
3.2 | - |
L-kynurenine | pH 8.0, 25°C | Thermus thermophilus | |
3.5 | - |
L-glutamine | pH 8.0, 25°C | Thermus thermophilus | |
6 | - |
L-tryptophan | pH 8.0, 25°C | Thermus thermophilus | |
18 | - |
L-histidine | pH 8.0, 25°C | Thermus thermophilus | |
19 | - |
L-aspartate | pH 8.0, 25°C | Thermus thermophilus | |
21 | - |
L-glutamate | pH 8.0, 25°C | Thermus thermophilus | |
25 | - |
2-oxoglutarate | pH 8.0, 25°C | Thermus thermophilus | |
37 | - |
L-serine | pH 8.0, 25°C | Thermus thermophilus | |
39 | - |
2-oxoglutarate | pH 8.0, 25°C | Thermus thermophilus | |
55 | - |
L-threonine | pH 8.0, 25°C | Thermus thermophilus | |
63 | - |
L-alanine | pH 8.0, 25°C | Thermus thermophilus | |
130 | - |
L-arginine | pH 8.0, 25°C | Thermus thermophilus | |
180 | - |
L-leucine | pH 8.0, 25°C | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42271, calculated, 2 * 42000, SDS-PAGE, N-terminal amino acid sequence | Thermus thermophilus |
42271 | - |
2 * 42271, calculated, 2 * 42000, SDS-PAGE, N-terminal amino acid sequence | Thermus thermophilus |
82000 | - |
gel filtration | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
expression in Escherichia coli | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
expression in Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + pyruvate | - |
Thermus thermophilus | 5-guanidino-2-oxopentanoate + L-alanine | - |
r | |
L-asparagine + phenylpyruvate | - |
Thermus thermophilus | 2-oxosuccinamate + L-phenylalanine | - |
r | |
L-aspartate + pyruvate | - |
Thermus thermophilus | oxaloacetate + L-alanine | - |
r | |
L-aspartate + pyruvate | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | oxaloacetate + L-alanine | - |
r | |
L-glutamate + phenylpyruvate | - |
Thermus thermophilus | 2-oxoglutarate + L-phenylalanine | - |
r | |
L-glutamine + pyruvate | - |
Thermus thermophilus | 2-oxoglutaramate + L-alanine | - |
? | |
L-histidine + pyruvate | - |
Thermus thermophilus | 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine | - |
? | |
L-histidine + pyruvate | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine | - |
? | |
L-kynurenine + pyruvate | - |
Thermus thermophilus | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine | - |
r | |
L-kynurenine + pyruvate | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine | - |
r | |
L-leucine + phenylpyruvate | - |
Thermus thermophilus | 4-methyl-2-oxopentanoate + L-phenylalanine | - |
r | |
L-methionine + phenylpyruvate | - |
Thermus thermophilus | 4-methylsulfanyl-2-oxobutanoate + L-phenylalanine | - |
r | |
L-methionine + phenylpyruvate | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | 4-methylsulfanyl-2-oxobutanoate + L-phenylalanine | - |
r | |
L-phenylalanine + 2-oxoglutarate | - |
Thermus thermophilus | ? | - |
? | |
L-phenylalanine + 4-hydroxyphenylpyruvate | - |
Thermus thermophilus | phenylpyruvate + L-tyrosine | - |
r | |
L-phenylalanine + 4-methylsulfanyl-2-oxobutanoate | - |
Thermus thermophilus | phenylpyruvate + L-methionine | - |
r | |
L-phenylalanine + indolepyruvate | - |
Thermus thermophilus | phenylpyruvate + L-tryptophan | - |
r | |
L-phenylalanine + pyruvate | - |
Thermus thermophilus | phenylpyruvate + L-alanine | - |
r | |
L-phenylalanine + pyruvate | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | phenylpyruvate + L-alanine | - |
r | |
L-serine + pyruvate | - |
Thermus thermophilus | 3-hydroxy-2-oxopropanoate + L-alanine | - |
r | |
L-threonine + pyruvate | - |
Thermus thermophilus | 3-hydroxy-2-oxobutanoate + L-alanine | - |
r | |
L-tryptophan + pyruvate | - |
Thermus thermophilus | indolepyruvate + L-alanine | - |
r | |
L-tyrosine + 2-oxoglutaramate | - |
Thermus thermophilus | 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamine | - |
r | |
phenylpyruvate + L-alanine | - |
Thermus thermophilus | L-phenylalanine + pyruvate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42271, calculated, 2 * 42000, SDS-PAGE, N-terminal amino acid sequence | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
L-threonine | pH 8.0, 25°C | Thermus thermophilus | |
0.016 | - |
L-aspartate | pH 8.0, 25°C | Thermus thermophilus | |
0.03 | - |
L-glutamate | pH 8.0, 25°C | Thermus thermophilus | |
0.12 | - |
L-arginine | pH 8.0, 25°C | Thermus thermophilus | |
0.13 | - |
L-asparagine | pH 8.0, 25°C | Thermus thermophilus | |
0.18 | - |
L-alanine | pH 8.0, 25°C | Thermus thermophilus | |
1.2 | - |
L-serine | pH 8.0, 25°C | Thermus thermophilus | |
1.3 | - |
L-histidine | pH 8.0, 25°C | Thermus thermophilus | |
1.5 | - |
L-leucine | pH 8.0, 25°C | Thermus thermophilus | |
3.1 | - |
2-oxoglutarate | pH 8.0, 25°C | Thermus thermophilus | |
15 | - |
pyruvate | pH 8.0, 25°C | Thermus thermophilus | |
130 | - |
phenylpyruvate | pH 8.0, 25°C | Thermus thermophilus | |
480 | - |
Indolepyruvate | pH 8.0, 25°C | Thermus thermophilus | |
590 | - |
L-phenylalanine | pH 8.0, 25°C | Thermus thermophilus | |
620 | - |
4-hydroxyphenylpyruvate | pH 8.0, 25°C | Thermus thermophilus | |
620 | - |
4-methylsulfanyl-2-oxobutanoate | pH 8.0, 25°C | Thermus thermophilus | |
830 | - |
L-glutamine | pH 8.0, 25°C | Thermus thermophilus | |
970 | - |
L-tyrosine | pH 8.0, 25°C | Thermus thermophilus | |
990 | - |
L-tryptophan | pH 8.0, 25°C | Thermus thermophilus | |
1100 | - |
L-kynurenine | pH 8.0, 25°C | Thermus thermophilus | |
1200 | - |
L-methionine | pH 8.0, 25°C | Thermus thermophilus |