Cloned (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Protein Variants | Comment | Organism |
---|---|---|
E32A | 1% of wild-type activity | Bacillus sp. (in: Bacteria) |
E32D | 65% of wild-type activity | Bacillus sp. (in: Bacteria) |
E32Q | 10% of wild-type activity | Bacillus sp. (in: Bacteria) |
K33A | 64% of wild-type activity | Bacillus sp. (in: Bacteria) |
V33A | 110% of wild-type activity | Bacillus sp. (in: Bacteria) |
Y31A | 0.4% of wild-type activity | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
D-alanine | pH 8.5, 50°C, E32D mtant enzyme | Bacillus sp. (in: Bacteria) | |
1.4 | - |
2-oxoglutarate | pH 8.5, 50°C, E32Q mutant enzyme | Bacillus sp. (in: Bacteria) | |
1.8 | - |
2-oxoglutarate | pH 8.5, 50°C, E32A mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.1 | - |
D-alanine | pH 8.5, 50°C, wild-type and E32Q mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.1 | - |
2-oxoglutarate | pH 8.5, 50°C, E32D mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.4 | - |
2-oxoglutarate | pH 8.5, 50°C, Y33A mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.5 | - |
2-oxoglutarate | pH 8.5, 50°C, wild-type enzyme | Bacillus sp. (in: Bacteria) | |
10.1 | - |
2-oxoglutarate | pH 8.5, 50°C, Y31A mutant enzyme | Bacillus sp. (in: Bacteria) | |
15.5 | - |
2-oxoisovalerate | pH 8.5, 50°C, V33A mutant enzyme | Bacillus sp. (in: Bacteria) | |
28 | - |
2-oxoisovalerate | pH 8.5, 50°C, wild-type enzyme | Bacillus sp. (in: Bacteria) | |
32.2 | - |
2-oxoisovalerate | pH 8.5, 50°C, K35A mutant enzyme | Bacillus sp. (in: Bacteria) | |
35.6 | - |
2-oxoglutarate | pH 8.5, 50°C, K35A mutant enzyme | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes, Resource Q, phenyl-superose | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + 2-oxoisovalerate | - |
Bacillus sp. (in: Bacteria) | pyruvate + D-valine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
D-alanine | pH 8.5, 50°C, Y31A mutant enzyme | Bacillus sp. (in: Bacteria) | |
1.3 | - |
D-alanine | pH 8.5, 50°C, E32A mutant enzyme | Bacillus sp. (in: Bacteria) | |
13.4 | - |
D-alanine | pH 8.5, 50°C, E32Q mutant enzyme | Bacillus sp. (in: Bacteria) | |
84.3 | - |
D-alanine | pH 8.5, 50°C, K35A mutant enzyme | Bacillus sp. (in: Bacteria) | |
85.6 | - |
D-alanine | pH 8.5, 50°C, E32D mutant enzyme | Bacillus sp. (in: Bacteria) | |
131 | - |
D-alanine | pH 8.5, 50°C, wild-type enzyme | Bacillus sp. (in: Bacteria) | |
145 | - |
D-alanine | pH 8.5, 50°C, V33A mutant enzyme | Bacillus sp. (in: Bacteria) |