Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
enzyme F-II | - |
Purification (Comment) | Organism |
---|---|
enzyme F-II | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + 2-oxobutanoate | - |
Pseudomonas sp. | pyruvate + 2-aminobutanoate | - |
r | |
L-alanine + 2-oxoglutarate | at 2% of the activity with 2-oxobutanoate | Pseudomonas sp. | pyruvate + L-glutamate | - |
r | |
L-alanine + 2-oxohexanoate | at 81% of the activity with 2-oxobutanoate | Pseudomonas sp. | pyruvate + 2-aminohexanoate | - |
r | |
L-alanine + 2-oxoisohexanoate | at 8% of the activity with 2-oxobutanoate | Pseudomonas sp. | pyruvate + L-leucine | - |
r | |
L-alanine + 2-oxoisopentanoate | at 10% of the activity with 2-oxobutanoate | Pseudomonas sp. | pyruvate + L-valine | - |
r | |
L-alanine + 2-oxopentanoate | at 108% of the activity with 2-oxobutanoate | Pseudomonas sp. | pyruvate + L-norvaline | - |
r | |
L-alanine + glyoxylate | 53% of the activity with 2-oxobutanoate | Pseudomonas sp. | pyruvate + glycine | - |
? | |
L-alanine + oxaloacetate | at 16% of the activity with 2-oxobutanoate | Pseudomonas sp. | pyruvate + L-aspartate | - |
r | |
pyruvate + L-2-aminobutanoate | preferred amino donor | Pseudomonas sp. | L-alanine + 2-oxobutanoate | - |
r | |
pyruvate + L-aspartate | 4% of the activity with L-2-aminobutanoate | Pseudomonas sp. | L-alanine + oxaloacetate | - |
? | |
pyruvate + L-glutamate | 4% of the activity with L-2-aminobutanoate | Pseudomonas sp. | L-alanine + 2-oxoglutarate | - |
r | |
pyruvate + L-isoleucine | 6% of the activity with L-2-aminobutanoate | Pseudomonas sp. | L-alanine + 3-methyl-2-oxopentanoate | - |
? | |
pyruvate + L-leucine | 16% of the activity with L-2-aminobutanoate | Pseudomonas sp. | alanine + 4-methyl-2-oxopentanoate | i.e. 2-oxoisohexanoate | r | |
pyruvate + L-norleucine | 31% of the activity with L-2-aminobutanoate | Pseudomonas sp. | L-alanine + 2-oxohexanoate | - |
? | |
pyruvate + L-norvaline | 84% of the activity with L-2-aminobutanoate | Pseudomonas sp. | L-alanine + 2-oxopentanoate | - |
r | |
pyruvate + L-phenylalanine | 4% of the activity with L-2-aminobutanoate | Pseudomonas sp. | L-alanine + 2-oxo-3-phenylpropanoate | - |
? | |
pyruvate + L-threonine | 3% of the activity with L-2-aminobutanoate | Pseudomonas sp. | L-alanine + 3-hydroxy-2-oxobutanoate | - |
? | |
pyruvate + L-valine | 8% of the activity with L-2-aminobutanoate | Pseudomonas sp. | L-alanine + 3-methyl-2-oxobutanoate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas sp. |