KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
2-oxoglutarate | pH 8.0, 50°C | Bacillus sp. (in: Bacteria) | |
3 | - |
L-aspartate | pH 8.0, 50°C | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
97000 | 98000 | gel filtration, sedimentation equilibrium | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Bacillus sp. (in: Bacteria) | - |
oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | Bacillus sp. (in: Bacteria) YM-2 | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) YM-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
220 | - |
- |
Bacillus sp. (in: Bacteria) |
Storage Stability | Organism |
---|---|
-20°C, protein concentration above 10 mg/ml | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Bacillus sp. (in: Bacteria) | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Bacillus sp. (in: Bacteria) YM-2 | oxaloacetate + L-glutamate | - |
? | |
L-cysteine sulfinic acid + 2-oxoglutarate | - |
Bacillus sp. (in: Bacteria) | 2-oxo-3-sulfinopropionic acid + L-glutamate | - |
ir | |
L-cysteine sulfinic acid + 2-oxoglutarate | - |
Bacillus sp. (in: Bacteria) YM-2 | 2-oxo-3-sulfinopropionic acid + L-glutamate | - |
ir | |
L-tryptophan + 2-oxoglutarate | 0.1% of the activity with aspartate | Bacillus sp. (in: Bacteria) | 3-indole-2-oxopropionic acid + L-glutamate | - |
? | |
L-tryptophan + 2-oxoglutarate | 0.1% of the activity with aspartate | Bacillus sp. (in: Bacteria) YM-2 | 3-indole-2-oxopropionic acid + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
1 h, pH 6.0-11.0, stable | Bacillus sp. (in: Bacteria) |
80 | - |
20 min, 35% loss of activity | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | 37°C, 1 h, stable | Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 1 mol of pyridoxal phosphate per mol of subunit | Bacillus sp. (in: Bacteria) |