Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
4 * 43000, SDS-PAGE | Methanothermobacter thermautotrophicus |
180000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Methanothermobacter thermautotrophicus | - |
oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | Methanothermobacter thermautotrophicus SF-4 | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Methanothermobacter thermautotrophicus | - |
strains FTF-INRA, DSM 3012 | - |
Methanothermobacter thermautotrophicus | - |
DSM 6457 | - |
Methanothermobacter thermautotrophicus SF-4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.8 | - |
purified enzyme | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Methanothermobacter thermautotrophicus | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Methanothermobacter thermautotrophicus SF-4 | oxaloacetate + L-glutamate | - |
? | |
L-cysteine + 2-oxoglutarate | - |
Methanothermobacter thermautotrophicus | 3-mercaptopyruvate + L-glutamate | - |
? | |
L-cysteine + 2-oxoglutarate | - |
Methanothermobacter thermautotrophicus SF-4 | 3-mercaptopyruvate + L-glutamate | - |
? | |
L-glutamate + 2-oxoglutarate | - |
Methanothermobacter thermautotrophicus | 2-oxoglutarate + L-glutamate | - |
? | |
L-glutamate + 2-oxoglutarate | - |
Methanothermobacter thermautotrophicus SF-4 | 2-oxoglutarate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 43000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Methanothermobacter thermautotrophicus |
70 | - |
strain FTF-INRA | Methanothermobacter thermautotrophicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 7.5, 1 h, gradual loss of activity above | Methanothermobacter thermautotrophicus |
70 | - |
pH 7.5, 1 h, stable up to | Methanothermobacter thermautotrophicus |
70 | - |
pH 7.5, 1 h, enzyme loses 65% of activity | Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Methanothermobacter thermautotrophicus |