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Literature summary for 2.5.1.91 extracted from

  • Zhang, H.; Li, Z.X.
    In vitro and in vivo characterization of a novel insect decaprenyl diphosphate synthase: a two-major step catalytic mechanism is proposed (2013), Biochem. Biophys. Res. Commun., 442, 105-111.
    View publication on PubMed
Search for more literature for 2.5.1.91

Cloned(Commentary)

Cloned (Comment) Organism
gene AgDPPS1, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of N-terminally GST-tagged enzyme in Escherichia coli strain BL21, coexpression with gene AgDPPS2 Aphis gossypii
gene AgDPPS2, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of N-terminally GST-tagged enzyme in Escherichia coli strain BL21, coexpression with gene AgDPPS1 Aphis gossypii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Aphis gossypii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46150
-
-
 Aphis gossypii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate Aphis gossypii
-
 7 diphosphate + all-trans-decaprenyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Aphis gossypii M9VRD0 gene AgDPPS2 encoding subunit 2
-
Aphis gossypii M9VU49 gene AgDPPS1 encoding subunit 1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography and ultrafiltration, tag cleavage by Factor Xa, followed by again affinity chromatography and ultrafiltration Aphis gossypii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate
-
 Aphis gossypii 7 diphosphate + all-trans-decaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate a major intermediate product (GGPP) is produced during the consecutive condensation reaction starting from FPP and IPP, leading to the formation of the final product (DPP) by adding six more IPPs. The two-major step catalytic mechanism might be a biochemical feature of insect DPPSs Aphis gossypii 7 diphosphate + all-trans-decaprenyl diphosphate
-
 ?
additional information no activity with dimethylallyl diphosphate Aphis gossypii ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 46150, subunit 1/AgDPPS1 + 1 x 46740, subunit 2/AgDPPS2, sequence calculation Aphis gossypii
More the two subunits are essential for the function of enzyme DPPS, since neither AgDPPS1 nor AgDPPS2 alone show catalytic activity Aphis gossypii

Synonyms

Synonyms Comment Organism
AgDPPS
-
 Aphis gossypii
decaprenyl diphosphate synthase 1
-
 Aphis gossypii
decaprenyl diphosphate synthase 2
-
 Aphis gossypii
decraprenyl diphosphate synthase
-
 Aphis gossypii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aphis gossypii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Aphis gossypii

pI Value

Organism Comment pI Value Maximum pI Value
Aphis gossypii sequence calculation
-
 6.28
Aphis gossypii sequence calculation
-
 8.13

General Information

General Information Comment Organism
evolution the enzyme contains six conserved motifs (from I to V), i.e., motif I (GKxxR), motif II (DDxxD(x)4RRG), motif III (GExxQ), motif IV (Y(x)6KT), motif V (FQLxDDxxD) and motif VI (K(x)4DxxxGxxTxPxL), occurring in all DPPS-1s examined, including insect, vertebrate, yeast and plant DPPS-1s, and bacterial DPPSs Aphis gossypii
physiological function the insect long-chain prenyl diphosphate synthase is responsible for the biosynthesis of the side-chain of ubiquinone-10, UQ-10, in Aphis gossypii Aphis gossypii