Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Mycobacterium tuberculosis | |
additional information | stimulation by detergent | Mycobacterium tuberculosis | |
Triton X-100 | maximum activity at 0.1% | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
neryl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
0.04 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
0.084 | - |
(2E,6E)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
0.089 | - |
isopentenyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
0.29 | - |
(2E,6Z)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
0.49 | - |
geranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | much less effective than Mg2+ | Mycobacterium tuberculosis | |
Mg2+ | maximum activity at 1 mM | Mycobacterium tuberculosis | |
Mn2+ | maximum activity at 0.1 mM, about 35% of the activity with Mg2+ | Mycobacterium tuberculosis | |
additional information | absolute requirement for divalent kations | Mycobacterium tuberculosis | |
Zn2+ | much less effective than Mg2+ | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | - |
Mycobacterium tuberculosis | 7 diphosphate + decaprenyl diphosphate | - |
? | |
(2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | diphosphate + octaprenyl diphosphate | plus, in decreasing order, nonaprenyl diphosphate, decaprenyl diphosphate, and heptaprenyl diphosphate | ? | |
(2E,6Z)-farnesyl diphosphate + isopentenyl diphosphate | highest catalytic efficiency | Mycobacterium tuberculosis | diphosphate + decaprenyl diphosphate | plus smaller amounts of intermediiates with shorter chain length | ? | |
geranyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | diphosphate + decaprenyl diphosphate | plus some farnesyl diphosphate | ? | |
neryl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | diphosphate + nonaprenyl diphosphate | plus some farnesyl diphosphate | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
0.052 | - |
neryl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
0.06 | - |
geranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
0.072 | - |
(2E,6E)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
0.08 | - |
isopentenyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
11.5 | - |
(2E,6Z)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.122 | - |
geranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
0.75 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
0.86 | - |
(2E,6E)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
0.89 | - |
isopentenyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
1.79 | - |
neryl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
39.65 | - |
(2E,6Z)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis |