Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain XL-1 Blue MRF' from vector pQE30-dmatsNeo | Neosartorya sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Neosartorya sp. | |
0.052 | - |
dimethylallyl diphosphate | pH 7.5, 37°C, recombinant enzyme | Neosartorya sp. | |
0.081 | - |
L-abrine | pH 7.5, 37°C, recombinant enzyme | Neosartorya sp. | |
0.238 | - |
L-tryptophan | pH 7.5, 37°C, recombinant enzyme | Neosartorya sp. | |
0.416 | - |
5-methyl-DL-tryptophan | pH 7.5, 37°C, recombinant enzyme | Neosartorya sp. | |
2.876 | - |
L-tryptophan methyl ester | pH 7.5, 37°C, recombinant enzyme | Neosartorya sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Neosartorya sp. | |
Mg2+ | required | Neosartorya sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
- |
Neosartorya sp. |
53000 | - |
- |
Neosartorya sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-tryptophan | Neosartorya sp. | - |
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neosartorya sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain XL-1 Blue MRF' by nickel affinity chromatography to homogeneity | Neosartorya sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
dimethylallyl diphosphate + L-tryptophan = diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | catalytic reaction mechanism involving Glu138, overview | Neosartorya sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 4-methyl-DL-tryptophan | - |
Neosartorya sp. | diphosphate + 7-(3-methylbut-2-enyl)-4-methyl-DL-tryptophan | - |
? | |
dimethylallyl diphosphate + 5-methoxy-DL-tryptophan | - |
Neosartorya sp. | diphosphate + 7-(3-methylbut-2-enyl)-5-methoxy-DL-tryptophan | - |
? | |
dimethylallyl diphosphate + 5-methyl-DL-tryptophan | - |
Neosartorya sp. | diphosphate + 7-(3-methylbut-2-enyl)-5-methyl-DL-tryptophan | - |
? | |
dimethylallyl diphosphate + 6-methyl-DL-tryptophan | - |
Neosartorya sp. | diphosphate + 7-(3-methylbut-2-enyl)-6-methyl-DL-tryptophan | - |
? | |
dimethylallyl diphosphate + L-abrine | - |
Neosartorya sp. | diphosphate + ? | - |
? | |
dimethylallyl diphosphate + L-tryptophan | - |
Neosartorya sp. | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan methyl ester | - |
Neosartorya sp. | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan methyl ester | - |
? | |
dimethylallyl diphosphate + N-acetyl-DL-tryptophan | - |
Neosartorya sp. | diphosphate + 7-(3-methylbut-2-enyl)-N-acetyl-DL-tryptophan | - |
? | |
dimethylallyl diphosphate + tryptamine | - |
Neosartorya sp. | diphosphate + 7-(3-methylbut-2-enyl)-tryptamine | - |
? | |
additional information | 7-DMATSNeo accepts not only L-tryptophan but also simple indole derivatives as substrates. The retention of the amino group of L-tryptophan appears to be an essential factor for molecular recognition by 7-DMATSNeo. No or poor activity with D-tryptophan, 5-hydroxy-L-tryptophan, indole-3-propionic acid, indole-3-pyruvic acid, DL-indole-3-lactic acid, glycyl-L-tryptophan, and cyclo(glycyl-L-tryptophan). No activity with 5-hydoroxy-L-tryptophan, 7-methyl-DL-tryptophan, 6-fluoro-DL-tryptophan, and 1-methyl-DL-tryptophan. Mass spectrometric product identification and analysis, overview | Neosartorya sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, about, recombinant His6-tagged enzyme, SDS-PAGE, x * 53000, about, sequence calculation | Neosartorya sp. |
Synonyms | Comment | Organism |
---|---|---|
7-dimethylallyl tryptophan synthase | - |
Neosartorya sp. |
7-DMATS | - |
Neosartorya sp. |
7-DMATSNeo | - |
Neosartorya sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Neosartorya sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
L-abrine | pH 7.5, 37°C, recombinant enzyme | Neosartorya sp. | |
0.092 | - |
dimethylallyl diphosphate | pH 7.5, 37°C, recombinant enzyme | Neosartorya sp. | |
0.108 | - |
L-tryptophan | pH 7.5, 37°C, recombinant enzyme | Neosartorya sp. | |
0.112 | - |
L-tryptophan methyl ester | pH 7.5, 37°C, recombinant enzyme | Neosartorya sp. | |
0.125 | - |
5-methyl-DL-tryptophan | pH 7.5, 37°C, recombinant enzyme | Neosartorya sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Neosartorya sp. |
General Information | Comment | Organism |
---|---|---|
additional information | analysis of the structural basis of the regioselective prenylation of L-tryptophan at the C7 position by comparison of the three-dimensional structural models of 7-DMATSNeo with FgaPT2 (4-DMATS), EC 2.5.1.34, from Aspergillus fumigatus. Construction of three-dimensional structural models of 7 DMATSNeo and FgaPT2 (4-DMATS). Docking studies of potential substrates, overview. Residues F129, L130, E138, F226, F243 are involved in binding of L-tryptophan, residue Y393 is involved in stabilization of the allylic cation via cation-Pi interactions. Residues R154, K239, Y241, R311, K313, K391, Y393, K454, and Y460 are involved in diphosphate coordination | Neosartorya sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
L-tryptophan methyl ester | pH 7.5, 37°C, recombinant enzyme | Neosartorya sp. | |
0.3 | - |
5-methyl-DL-tryptophan | pH 7.5, 37°C, recombinant enzyme | Neosartorya sp. | |
0.47 | - |
L-tryptophan | pH 7.5, 37°C, recombinant enzyme | Neosartorya sp. | |
0.542 | - |
L-abrine | pH 7.5, 37°C, recombinant enzyme | Neosartorya sp. | |
1.76 | - |
dimethylallyl diphosphate | pH 7.5, 37°C, recombinant enzyme | Neosartorya sp. |