Cloned (Comment) | Organism |
---|---|
Lambda ZAP Express system | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
additional information | 4 insertion allels (m1-m4) of camouflage1 (cf1) mutant, mutant develops nonclonal, yellow-green sectors in leaves based on bundle sheath cell-specific death, yellow mutant regions show reduced levels of chlorophyll a + b, and total carotenoids, reduced photosynthesis and stomatal conductance compared to wild type and green regions of the mutant, constant light suppresses the cf1 sector formation, sectors only form during a limited time of leaf development, underlying is a decreased enzyme activity and increased levels of the substrate in green and yellow regions, yellow regions show an additional reduction in catalase activity | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | fusion enzyme with yellow fluorescent protein (YFP), transformed in Vicia faba leaves for localization analysis | Zea mays | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 porphobilinogen + H2O | Zea mays | - |
hydroxymethylbilane + 4 NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
maize | - |
Purification (Comment) | Organism |
---|---|
leaves of 8 week old field grown plants are ground in liquid nitrogen and extracted with 25 mM NaP buffer, pH 8.0, containing 1 mM EDTA, 5 mM DTT, and 0.5 mM phenylmethanesulfonylfluoride, centrifuged, supernatant taken for enzyme assay | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | in developing ears | Zea mays | - |
leaf | mature and etiolated leaves | Zea mays | - |
root | seedling roots | Zea mays | - |
tassel | in developing tassels | Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
cf1 mutant tissue shows 30% reduced activity (about 0.7 units/mg) compared to wild type (about 2.5 units/mg) in green and yellow leaf tissue, consistent with reduced enzyme abundance, 13fold substrate accumulation | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 porphobilinogen + H2O | - |
Zea mays | hydroxymethylbilane + 4 NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PBGD | - |
Zea mays |
porphobilinogen deaminase | - |
Zea mays |
Organism | Comment | Expression |
---|---|---|
Zea mays | cf1 mutation show strongly decreased expression compared to wild type | down |
General Information | Comment | Organism |
---|---|---|
malfunction | camouflage1 (cf1) mutant develops nonclonal, yellow-green sectors in leaves based on bundle sheath cell-specific death, yellow mutant regions show reduced levels of chlorophyll a + b, and total carotenoids, reduced photosynthesis and stomatal conductance compared to wild type and green regions of the mutant, constant light suppresses the cf1 sector formation, sectors only form during a limited time of leaf development, underlying is a decreased enzyme activity and increased levels of the substrate in green and yellow regions, yellow regions show an additional reduction in catalase activity | Zea mays |
physiological function | early step in chlorophyll and heme biosynthesis | Zea mays |