Application | Comment | Organism |
---|---|---|
medicine | evidence that inhibitors of enzyme could be effective therapeutic agents in treatment of many human cancers | Homo sapiens |
medicine | evidence that inhibitors of enzyme could be effective therapeutic agents in treatment of many human cancers | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
enzyme produced using an Sf9 cell overexpression | Homo sapiens |
translationally coupled two-cistron expression system for enzyme in Escherichia coli | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
a new crystalline form of enzyme for the mutant truncated 10 residues at the C-terminus | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | - |
Homo sapiens |
additional information | three beta-subunit C-terminal truncation mutants, deletion of 5, 10 and 14 residues, the overall catalytic efficiency of enzyme decreases gradually with increasing C-terminal deletion | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KKSKTKCVIM | - |
Rattus norvegicus | |
KKTKSKCVIM | - |
Rattus norvegicus | |
additional information | enzyme inhibited by peptides containing the C-terminal CAAX sequence | Rattus norvegicus | |
NH2-KTKCVFM | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | comparison of Km of mutant enzymes | Homo sapiens | |
additional information | - |
additional information | comparison of Km of mutant enzymes | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
alpha,beta, 1 * 50000 + 1 * 44000, SDS-PAGE, 1 * 46200 + 1 * 487000, mass spectroscopy | Rattus norvegicus |
46200 | - |
alpha,beta, 1 * 50000 + 1 * 44000, SDS-PAGE, 1 * 46200 + 1 * 487000, mass spectroscopy | Rattus norvegicus |
50000 | - |
alpha,beta, 1 * 50000 + 1 * 44000, SDS-PAGE, 1 * 46200 + 1 * 487000, mass spectroscopy | Rattus norvegicus |
487000 | - |
alpha,beta, 1 * 50000 + 1 * 44000, SDS-PAGE, 1 * 46200 + 1 * 487000, mass spectroscopy | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + protein-cysteine | Homo sapiens | - |
S-farnesyl protein + diphosphate | - |
? | |
farnesyl diphosphate + protein-cysteine | Rattus norvegicus | - |
S-farnesyl protein + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate | kinetic mechanism | Rattus norvegicus | |
farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate | enzyme constitutes the protein prenyltransferase family of enzymes | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + protein-cysteine | - |
Homo sapiens | diphosphate + S-farnesyl protein | - |
? | |
farnesyl diphosphate + protein-cysteine | posttranslational lipid modification in which a 15-carbon farnesyl isoprenoid is linked via a thioether bond to specific cysteine residues of proteins, the reactive cysteine is located in the C-terminal Ca1a2X motif in which C is the modified cysteine, a1 and a2 are often an aliphatic residue, and X is Ser, Met, Ala or Gln | Rattus norvegicus | diphosphate + S-farnesyl protein | - |
? | |
farnesyl diphosphate + protein-cysteine | substrate are: Ras superfamiliy and Ras-related proteins for example Rap, Rab, Rac and Ral, large G proteins, nuclear lamins, rhodopsin kinase and the retinal cGMP phosphodiesterase | Rattus norvegicus | diphosphate + S-farnesyl protein | - |
? | |
farnesyl diphosphate + protein-cysteine | - |
Homo sapiens | S-farnesyl protein + diphosphate | - |
? | |
farnesyl diphosphate + protein-cysteine | - |
Rattus norvegicus | S-farnesyl protein + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | alpha,beta, 1 * 50000 + 1 * 44000, SDS-PAGE, 1 * 46200 + 1 * 487000, mass spectroscopy | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | comparison of kcat of mutant enzymes | Homo sapiens | |
additional information | - |
additional information | comparison of kcat of mutant enzymes | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00012 | - |
NH2-KTKCVFM | - |
Rattus norvegicus | |
0.0013 | - |
KKSKTKCVIM | - |
Rattus norvegicus |