Literature summary for 2.5.1.18 extracted from

Osman, W.H.W.; Mikami, B.; Saka, N.; Kondo, K.; Lin, M.I.; Nagata, T.; Katahira, M.
Identification of key residues for activities of atypical glutathione S-transferase of Ceriporiopsis subvermispora, a selective degrader of lignin in woody biomass, by crystallography and functional mutagenesis (2019), Int. J. Biol. Macromol., 132, 222-229 .

Search for more literature for 2.5.1.18

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in GSH-free and -bound forms, using 20% (w/v) PEG8000, 100 mM HEPES-HCl (pH 7.5), 200 mM ammonium sulfate, and 20% (v/v) 2-propanol at 4°C	Gelatoporia subvermispora

Protein Variants

Protein Variants	Comment	Organism
N22A	the mutant shows reduced activity towards 1-chloro-2,4-dinitrobenzene and increased activity towards 5-chloromethylfluorescein diacetate compared to the wild type enzyme	Gelatoporia subvermispora
N24A	the mutant shows no activity towards 1-chloro-2,4-dinitrobenzene and alpha-O-(beta-methylumbelliferyl)acetovanillone and increased activity towards 5-chloromethylfluorescein diacetate and cumene hydroperoxide compared to the wild type enzyme	Gelatoporia subvermispora
Y46A	the mutant shows no activity towards 1-chloro-2,4-dinitrobenzene and increased activity towards 5-chloromethylfluorescein diacetate compared to the wild type enzyme	Gelatoporia subvermispora

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00003	-	4-methylumbelliferyl acetate	mutant enzyme N24A, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.00022	-	5-chloromethylfluorescein diacetate	mutant enzyme N24A, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.00033	-	5-chloromethylfluorescein diacetate	mutant enzyme N22A, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.00049	-	5-chloromethylfluorescein diacetate	mutant enzyme Y46A, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.00094	-	4-methylumbelliferyl acetate	wild type enzyme, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.01162	-	5-chloromethylfluorescein diacetate	wild type enzyme, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.01461	-	alpha-O-(beta-methylumbelliferyl)acetovanillone	wild type enzyme, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.11	-	cumene hydroperoxide	wild type enzyme, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.412	-	1-chloro-2,4-dinitrobenzene	mutant enzyme N22A, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.602	-	1-chloro-2,4-dinitrobenzene	wild type enzyme, pH and temperature not specified in the publication	Gelatoporia subvermispora
5.3	6	cumene hydroperoxide	mutant enzyme N24A, pH and temperature not specified in the publication	Gelatoporia subvermispora

Organism

Organism	UniProt	Comment	Textmining
Gelatoporia subvermispora	M2QJS8	-	-

Purification (Commentary)

Purification (Comment)	Organism
anion exchange chromatography and Superdex S200 gel filtration	Gelatoporia subvermispora

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathione + 1-chloro-2,4-dinitrobenzene	-	Gelatoporia subvermispora	chloride + 2,4-dinitrophenyl-glutathione	-	?
glutathione + 4-methylumbelliferyl acetate	-	Gelatoporia subvermispora	?	-	?
glutathione + 5-chloromethylfluorescein diacetate	-	Gelatoporia subvermispora	?	-	?
glutathione + alpha-O-(beta-methylumbelliferyl)acetovanillone	-	Gelatoporia subvermispora	?	-	?
glutathione + cumene hydroperoxide	-	Gelatoporia subvermispora	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 31000, SDS-PAGE	Gelatoporia subvermispora
homodimer	2 * 29830, calculated from amino acid sequence	Gelatoporia subvermispora

Synonyms

Synonyms	Comment	Organism
glutathione S-transferase	-	Gelatoporia subvermispora
GST83044	-	Gelatoporia subvermispora

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.000000451	-	alpha-O-(beta-methylumbelliferyl)acetovanillone	wild type enzyme, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.00000163	-	4-methylumbelliferyl acetate	mutant enzyme N24A, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.000146	-	5-chloromethylfluorescein diacetate	mutant enzyme Y46A, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.000321	-	5-chloromethylfluorescein diacetate	mutant enzyme N24A, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.0007	-	cumene hydroperoxide	mutant enzyme N24A, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.000714	-	5-chloromethylfluorescein diacetate	wild type enzyme, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.000906	-	5-chloromethylfluorescein diacetate	mutant enzyme N22A, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.00333	-	4-methylumbelliferyl acetate	wild type enzyme, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.0678	-	1-chloro-2,4-dinitrobenzene	mutant enzyme N22A, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.18	-	1-chloro-2,4-dinitrobenzene	wild type enzyme, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.21	-	cumene hydroperoxide	wild type enzyme, pH and temperature not specified in the publication	Gelatoporia subvermispora

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.0000309	-	alpha-O-(beta-methylumbelliferyl)acetovanillone	wild type enzyme, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.05433	-	4-methylumbelliferyl acetate	mutant enzyme N24A, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.06145	-	5-chloromethylfluorescein diacetate	wild type enzyme, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.131	-	cumene hydroperoxide	mutant enzyme N24A, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.165	-	1-chloro-2,4-dinitrobenzene	mutant enzyme N22A, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.298	-	5-chloromethylfluorescein diacetate	mutant enzyme Y46A, pH and temperature not specified in the publication	Gelatoporia subvermispora
0.299	-	1-chloro-2,4-dinitrobenzene	wild type enzyme, pH and temperature not specified in the publication	Gelatoporia subvermispora
1.46	-	5-chloromethylfluorescein diacetate	mutant enzyme N24A, pH and temperature not specified in the publication	Gelatoporia subvermispora
1.91	-	cumene hydroperoxide	wild type enzyme, pH and temperature not specified in the publication	Gelatoporia subvermispora
2.75	-	5-chloromethylfluorescein diacetate	mutant enzyme N22A, pH and temperature not specified in the publication	Gelatoporia subvermispora
3.54	-	4-methylumbelliferyl acetate	wild type enzyme, pH and temperature not specified in the publication	Gelatoporia subvermispora

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Release 2023.1 (January 2023)