Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Salix babylonica |
Protein Variants | Comment | Organism |
---|---|---|
I55T | the mutant of isoform GSTU6 shows strongly decreased activity compared to the wild type enzyme | Salix babylonica |
T53I | the mutant of isoform GSTU7 shows strongly increased activity compared to the wild type enzyme | Salix babylonica |
T53V | the mutant of isoform GSTU7 shows strongly increased activity compared to the wild type enzyme | Salix babylonica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
glutathione | isoform GSTU3, at pH 6.5 and 25°C | Salix babylonica | |
0.141 | - |
glutathione | isoform GSTU6, at pH 6.5 and 25°C | Salix babylonica | |
0.146 | - |
glutathione | isoform GSTU4, at pH 6.5 and 25°C | Salix babylonica | |
0.156 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU10, at pH 6.5 and 25°C | Salix babylonica | |
0.201 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU1, at pH 6.5 and 25°C | Salix babylonica | |
0.208 | - |
glutathione | isoform GSTU9, at pH 6.5 and 25°C | Salix babylonica | |
0.24 | - |
glutathione | isoform GSTU5, at pH 6.5 and 25°C | Salix babylonica | |
0.262 | - |
glutathione | isoform GSTU13, at pH 6.5 and 25°C | Salix babylonica | |
0.284 | - |
glutathione | isoform GSTU10, at pH 6.5 and 25°C | Salix babylonica | |
0.333 | - |
glutathione | isoform GSTU12, at pH 6.5 and 25°C | Salix babylonica | |
0.473 | - |
glutathione | isoform GSTU1, at pH 6.5 and 25°C | Salix babylonica | |
0.491 | - |
glutathione | isoform GSTU14, at pH 6.5 and 25°C | Salix babylonica | |
0.732 | - |
glutathione | isoform GSTU11, at pH 6.5 and 25°C | Salix babylonica | |
0.893 | - |
glutathione | isoform GSTU8, at pH 6.5 and 25°C | Salix babylonica | |
0.996 | - |
glutathione | isoform GSTU2, at pH 6.5 and 25°C | Salix babylonica | |
1.003 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU3, at pH 6.5 and 25°C | Salix babylonica | |
1.183 | - |
glutathione | isoform GSTU7, at pH 6.5 and 25°C | Salix babylonica | |
1.273 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU13, at pH 6.5 and 25°C | Salix babylonica | |
2.074 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU7, at pH 6.5 and 25°C | Salix babylonica | |
2.144 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU12, at pH 6.5 and 25°C | Salix babylonica | |
2.266 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU6, at pH 6.5 and 25°C | Salix babylonica | |
2.364 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU5, at pH 6.5 and 25°C | Salix babylonica | |
2.628 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU8, at pH 6.5 and 25°C | Salix babylonica | |
2.785 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU9, at pH 6.5 and 25°C | Salix babylonica | |
3.372 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU11, at pH 6.5 and 25°C | Salix babylonica | |
4.269 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU14, at pH 6.5 and 25°C | Salix babylonica | |
4.819 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU2, at pH 6.5 and 25°C | Salix babylonica | |
5.121 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU4, at pH 6.5 and 25°C | Salix babylonica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salix babylonica | A0A4Y5QZW9 | - |
- |
Salix babylonica | A0A4Y5QZX0 | - |
- |
Salix babylonica | A0A4Y5QZX2 | - |
- |
Salix babylonica | A0A4Y5QZX3 | - |
- |
Salix babylonica | A0A4Y5QZX9 | - |
- |
Salix babylonica | A0A4Y5QZZ8 | - |
- |
Salix babylonica | A0A4Y5R032 | - |
- |
Salix babylonica | A0A4Y5R087 | - |
- |
Salix babylonica | A0A4Y5R0B2 | - |
- |
Salix babylonica | A0A4Y5R0C1 | - |
- |
Salix babylonica | A0A4Y5R0K8 | - |
- |
Salix babylonica | A0A4Y5R0L5 | - |
- |
Salix babylonica | A0A4Y5R2M7 | - |
- |
Salix babylonica | A0A4Y5R2N2 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-Sepharose column chromatography | Salix babylonica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bud | - |
Salix babylonica | - |
flower | - |
Salix babylonica | - |
flower | highest expression | Salix babylonica | - |
flower | high expression | Salix babylonica | - |
leaf | - |
Salix babylonica | - |
leaf | highest expression | Salix babylonica | - |
phloem | - |
Salix babylonica | - |
phloem | high expression | Salix babylonica | - |
root | - |
Salix babylonica | - |
root | highest expression | Salix babylonica | - |
root | high expression | Salix babylonica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1,2-dichloro-4-nitrobenzene | - |
Salix babylonica | ? | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Salix babylonica | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
glutathione + 4-nitrobenzyl chloride | - |
Salix babylonica | chloride + 4-nitrobenzyl-glutathione | - |
? | |
glutathione + 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole | - |
Salix babylonica | ? | - |
? | |
glutathione + cumene hydroperoxide | - |
Salix babylonica | ? | - |
? | |
glutathione + fluorodifen | - |
Salix babylonica | ? | - |
? | |
additional information | no activity with 1,2-dichloro-4-nitrobenzene, dehydroascorbic acid, 4-nitrobenzyl chloride and cumene hydroperoxide | Salix babylonica | ? | - |
- |
|
additional information | no activity with dehydroascorbic acid and cumene hydroperoxide | Salix babylonica | ? | - |
- |
|
additional information | no activity with dehydroascorbic acid, 1,2-dichloro-4-nitrobenzene and cumene hydroperoxide | Salix babylonica | ? | - |
- |
|
additional information | no activity with dehydroascorbic acid, 4-nitrobenzyl chloride and cumene hydroperoxide | Salix babylonica | ? | - |
- |
|
additional information | no activity with dehydroascorbic acid, 4-nitrobenzyl chloride, 1,2-dichloro-4-nitrobenzene and cumene hydroperoxide | Salix babylonica | ? | - |
- |
|
additional information | no activity with fluorodifen, 1,2-dichloro-4-nitrobenzene, dehydroascorbic acid, 4-nitrobenzyl chloride and cumene hydroperoxide | Salix babylonica | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 26100, calculated from amino acid sequence | Salix babylonica |
? | x * 24756, calculated from amino acid sequence | Salix babylonica |
? | x * 24893, calculated from amino acid sequence | Salix babylonica |
? | x * 25014, calculated from amino acid sequence | Salix babylonica |
? | x * 25172, calculated from amino acid sequence | Salix babylonica |
? | x * 25262, calculated from amino acid sequence | Salix babylonica |
? | x * 25300, calculated from amino acid sequence | Salix babylonica |
? | x * 25340, calculated from amino acid sequence | Salix babylonica |
? | x * 25401, calculated from amino acid sequence | Salix babylonica |
? | x * 25493, calculated from amino acid sequence | Salix babylonica |
? | x * 25536, calculated from amino acid sequence | Salix babylonica |
? | x * 25567, calculated from amino acid sequence | Salix babylonica |
? | x * 25680, calculated from amino acid sequence | Salix babylonica |
? | x * 26434, calculated from amino acid sequence | Salix babylonica |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Salix babylonica |
GST | - |
Salix babylonica |
GSTU1 | isoform | Salix babylonica |
GSTU10 | isoform | Salix babylonica |
GSTU11 | isoform | Salix babylonica |
GSTU12 | isoform | Salix babylonica |
GSTU13 | isoform | Salix babylonica |
GSTU14 | isoform | Salix babylonica |
GSTU2 | isoform | Salix babylonica |
GSTU3 | isoform | Salix babylonica |
GSTU4 | isoform | Salix babylonica |
GSTU5 | isoform | Salix babylonica |
GSTU6 | isoform | Salix babylonica |
GSTU7 | isoform | Salix babylonica |
GSTU8 | isoform | Salix babylonica |
GSTU9 | isoform | Salix babylonica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.804 | - |
glutathione | isoform GSTU7, at pH 6.5 and 25°C | Salix babylonica | |
1.197 | - |
glutathione | isoform GSTU8, at pH 6.5 and 25°C | Salix babylonica | |
1.314 | - |
glutathione | isoform GSTU12, at pH 6.5 and 25°C | Salix babylonica | |
1.415 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU7, at pH 6.5 and 25°C | Salix babylonica | |
1.934 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU12, at pH 6.5 and 25°C | Salix babylonica | |
2.061 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU8, at pH 6.5 and 25°C | Salix babylonica | |
2.496 | - |
glutathione | isoform GSTU2, at pH 6.5 and 25°C | Salix babylonica | |
2.963 | - |
glutathione | isoform GSTU4, at pH 6.5 and 25°C | Salix babylonica | |
3.376 | - |
glutathione | isoform GSTU5, at pH 6.5 and 25°C | Salix babylonica | |
6.056 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU1, at pH 6.5 and 25°C | Salix babylonica | |
6.283 | - |
glutathione | isoform GSTU1, at pH 6.5 and 25°C | Salix babylonica | |
7.391 | - |
glutathione | isoform GSTU10, at pH 6.5 and 25°C | Salix babylonica | |
7.564 | - |
glutathione | isoform GSTU9, at pH 6.5 and 25°C | Salix babylonica | |
7.782 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU5, at pH 6.5 and 25°C | Salix babylonica | |
11.4 | - |
glutathione | isoform GSTU13, at pH 6.5 and 25°C | Salix babylonica | |
12.281 | - |
glutathione | isoform GSTU14, at pH 6.5 and 25°C | Salix babylonica | |
14.948 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU13, at pH 6.5 and 25°C | Salix babylonica | |
16.183 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU10, at pH 6.5 and 25°C | Salix babylonica | |
16.302 | - |
glutathione | isoform GSTU11, at pH 6.5 and 25°C | Salix babylonica | |
16.483 | - |
glutathione | isoform GSTU6, at pH 6.5 and 25°C | Salix babylonica | |
16.714 | - |
glutathione | isoform GSTU3, at pH 6.5 and 25°C | Salix babylonica | |
21.06 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU9, at pH 6.5 and 25°C | Salix babylonica | |
22.472 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU11, at pH 6.5 and 25°C | Salix babylonica | |
24.482 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU4, at pH 6.5 and 25°C | Salix babylonica | |
28.819 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU3, at pH 6.5 and 25°C | Salix babylonica | |
41.823 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU14, at pH 6.5 and 25°C | Salix babylonica | |
112.987 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU6, at pH 6.5 and 25°C | Salix babylonica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Salix babylonica | calculated from amino acid sequence | - |
5.1 |
Salix babylonica | calculated from amino acid sequence | - |
5.2 |
Salix babylonica | calculated from amino acid sequence | - |
5.3 |
Salix babylonica | calculated from amino acid sequence | - |
5.5 |
Salix babylonica | calculated from amino acid sequence | - |
5.8 |
Salix babylonica | calculated from amino acid sequence | - |
5.9 |
Salix babylonica | calculated from amino acid sequence | - |
6.2 |
Salix babylonica | calculated from amino acid sequence | - |
6.4 |
Salix babylonica | calculated from amino acid sequence | - |
6.6 |
Salix babylonica | calculated from amino acid sequence | - |
6.9 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.679 | - |
glutathione | isoform GSTU7, at pH 6.5 and 25°C | Salix babylonica | |
0.682 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU7, at pH 6.5 and 25°C | Salix babylonica | |
0.784 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU8, at pH 6.5 and 25°C | Salix babylonica | |
0.902 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU12, at pH 6.5 and 25°C | Salix babylonica | |
1.325 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU2, at pH 6.5 and 25°C | Salix babylonica | |
1.34 | - |
glutathione | isoform GSTU8, at pH 6.5 and 25°C | Salix babylonica | |
2.506 | - |
glutathione | isoform GSTU2, at pH 6.5 and 25°C | Salix babylonica | |
3.292 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU5, at pH 6.5 and 25°C | Salix babylonica | |
3.947 | - |
glutathione | isoform GSTU12, at pH 6.5 and 25°C | Salix babylonica | |
4.781 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU4, at pH 6.5 and 25°C | Salix babylonica | |
6.664 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU11, at pH 6.5 and 25°C | Salix babylonica | |
7.562 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU9, at pH 6.5 and 25°C | Salix babylonica | |
9.797 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU14, at pH 6.5 and 25°C | Salix babylonica | |
11.472 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU13, at pH 6.5 and 25°C | Salix babylonica | |
13.277 | - |
glutathione | isoform GSTU1, at pH 6.5 and 25°C | Salix babylonica | |
14.073 | - |
glutathione | isoform GSTU5, at pH 6.5 and 25°C | Salix babylonica | |
20.296 | - |
glutathione | isoform GSTU4, at pH 6.5 and 25°C | Salix babylonica | |
22.285 | - |
glutathione | isoform GSTU11, at pH 6.5 and 25°C | Salix babylonica | |
25.022 | - |
glutathione | isoform GSTU14, at pH 6.5 and 25°C | Salix babylonica | |
26.053 | - |
glutathione | isoform GSTU10, at pH 6.5 and 25°C | Salix babylonica | |
28.733 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU3, at pH 6.5 and 25°C | Salix babylonica | |
30.102 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU1, at pH 6.5 and 25°C | Salix babylonica | |
36.365 | - |
glutathione | isoform GSTU9, at pH 6.5 and 25°C | Salix babylonica | |
43.529 | - |
glutathione | isoform GSTU13, at pH 6.5 and 25°C | Salix babylonica | |
49.862 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU6, at pH 6.5 and 25°C | Salix babylonica | |
116.572 | - |
glutathione | isoform GSTU6, at pH 6.5 and 25°C | Salix babylonica | |
119.3 | - |
glutathione | isoform GSTU3, at pH 6.5 and 25°C | Salix babylonica | |
7104 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU10, at pH 6.5 and 25°C | Salix babylonica |