Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 1 mM dithiothreitol stimulates activity of the Ure2p mutant A122C | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
A122C | the single-site mutation A122C and N124A/V (but not N124S/Y/C) restores the GST activity of Ure2p protein toward 1-chloro-2,4-dinitrobenzene, while causing a substantial reduction in glutathione peroxidase activity | Saccharomyces cerevisiae |
N124V | the single-site mutation A122C and N124A/V (but not N124S/Y/C) restores the GST activity of Ure2p protein toward 1-chloro-2,4-dinitrobenzene, while causing a substantial reduction in glutathione peroxidase activity | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
glutathione | Ure2p mutant A122C, at 25°C and pH 8.0, in the presence of 1 mM dithiothreitol | Saccharomyces cerevisiae | |
5.8 | - |
glutathione | Ure2p mutant N124V, at 25°C and pH 8.0 | Saccharomyces cerevisiae | |
7.2 | - |
1-chloro-2,4-dinitrobenzene | Ure2p mutant A122C, at 25°C and pH 8.0, in the presence of 1 mM dithiothreitol | Saccharomyces cerevisiae | |
29.2 | - |
1-chloro-2,4-dinitrobenzene | Ure2p mutant N124V, at 25°C and pH 8.0 | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
the single-site mutation A122C and N124A/V (but not N124S/Y/C) restores the GST activity of Ure2p protein toward 1-chloro-2,4-dinitrobenzene, while causing a substantial reduction in glutathione peroxidase activity | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Saccharomyces cerevisiae | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ure2p mutant A122C | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
Ure2p mutant A122C | Saccharomyces cerevisiae |
42 | - |
Ure2p mutant N124V | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
glutathione | Ure2p mutant A122C, at 25°C and pH 8.0, in the presence of 1 mM dithiothreitol | Saccharomyces cerevisiae | |
1.7 | - |
glutathione | Ure2p mutant N124V, at 25°C and pH 8.0 | Saccharomyces cerevisiae |