Cloned (Comment) | Organism |
---|---|
cloned into the pQE-30 expression vector, and overexpressed in Escherichia coli M15 (pREP4) | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S] cluster | presence of the predicted [4Fe-4S] cluster. Iron and sulfide analysis yields 2.4 irons and 2.8 sulfides per monomer of MqnE, indicating incomplete reconstitution of the cluster | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3-[(1-carboxyvinyl)oxy]benzoate + H2O | Thermus thermophilus | the enzyme is part of the futalosine pathway for menaquinone biosynthesis | 6-amino-6-deoxyfutalosine + L-methionine + bicarbonate | 6-amino-6-deoxyfutalosine i.e. 3-[3-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]propanoyl]benzoate | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q5SK48 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3-[(1-carboxyvinyl)oxy]benzoate + H2O | - |
Thermus thermophilus | 6-amino-6-deoxyfutalosine + L-methionine + bicarbonate | 6-amino-6-deoxyfutalosine i.e. 3-[3-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]propanoyl]benzoate | ? | |
S-adenosyl-L-methionine + 3-[(1-carboxyvinyl)oxy]benzoate + H2O | the enzyme is part of the futalosine pathway for menaquinone biosynthesis | Thermus thermophilus | 6-amino-6-deoxyfutalosine + L-methionine + bicarbonate | 6-amino-6-deoxyfutalosine i.e. 3-[3-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]propanoyl]benzoate | ? |
Synonyms | Comment | Organism |
---|---|---|
AFL synthase | - |
Thermus thermophilus |
aminofutalosine synthase | - |
Thermus thermophilus |
MqnE | - |
Thermus thermophilus |
TTHA0804 | locus name | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | the enzyme is a member of the AdoMet radical family. S-Adenosyl-L-methionine acts as both a radical generator and as the source of the transferred adenosyl group | Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is part of the futalosine pathway for menaquinone biosynthesis | Thermus thermophilus |