Cloned (Comment) | Organism |
---|---|
screening for squalene synthase-like genes, identification of gene SSL-1, DNA and amino acid sequence determmination and analysis, co-expression with SSL-2 leads to30fold increased squalene production | Botryococcus braunii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (2E,6E)-farnesyl diphosphate | Botryococcus braunii | - |
presqualene diphosphate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Botryococcus braunii | G0Y286 | race B | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (2E,6E)-farnesyl diphosphate | - |
Botryococcus braunii | presqualene diphosphate + diphosphate | - |
? | |
2 (2E,6E)-farnesyl diphosphate | condensation of two molecules of farnesyl diphosphate | Botryococcus braunii | presqualene diphosphate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
squalene synthase-like | - |
Botryococcus braunii |
SSL | - |
Botryococcus braunii |
General Information | Comment | Organism |
---|---|---|
evolution | SSL_1 belongs to the family of squalene synthase-like enzymes. Triterpene metabolism in Botryococcus braunii operates differently from that in other organisms. While squalene synthase, an ancient and likely progenitor to the other Botryococcus triterpene synthases, catalyzes a two-step reaction within a single enzyme unit without intermediate release, yet in Botryococcus braunii, these activities appear to have separated and evolved interdependently for specialized greater triterpene oil production into SSL-1 an SSL-2 | Botryococcus braunii |
metabolism | SSL-1 catalyzes the biosynthesis of presqualene diphosphate. The product presqualene diphosphate is further converted to botryococcene by SSL-3 or to squalene by SSL-2.. While squalene synthase, an ancient and likely progenitor to the other Botryococcus triterpene synthases, catalyzes a two-step reaction within a single enzyme unit without intermediate release, yet in Botryococcus braunii, these activities appear to have separated and evolved interdependently for specialized greater triterpene oil production | Botryococcus braunii |
additional information | bisfarnesyl ether biosynthesis from (2E,6E)-farnesyl diphosphate by SSL-2, overview | Botryococcus braunii |