Cloned (Comment) | Organism |
---|---|
coexpression of the uracil phosphoribosyltransferase gene with a chimeric human nerve growth factor receptor/yeast cytosine deaminase fusion gene, using a single retroviral vector, augments cytotoxicity of transduced human T cells, CEM cells, exposed to 5-fluorocytosine | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | sensitivity of cells to 5-FU can be further increased by expression of UPRT, useful in an approach for the management of graft-versus-host disease occurring after allogeneic hematopoietic cell transplantation by genetical engineering of donor T lymphocytes to express a suicide gene, i.e. the Herpes simplex virus thymidine kinase HSV-tk gene, to facilitate negative selection, combination of UPRT expression with expression of cytosine deaminase, an alternative gene for negative selection, converting 5-fluorocytosine to the toxic metabolite 5-fluorouracil, a construct NG/CDiU expressing UPRT and NG/CD, using a bicistronic message, provides the greatest UPRT activity and killing, overview | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
gene UPRT | - |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharomyces cerevisiae | 5-fluoro-UMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UPRT | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |