General Stability | Organism |
---|---|
enzyme is extremly unstable, UMP stabilizes during storage | Candida albicans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0705 | - |
Uracil | - |
Candida albicans | |
0.186 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Candida albicans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Candida albicans | |
Mg2+ | optimal concentration : 2 mM | Candida albicans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 47000 + x * 38000, SDS-PAGE | Candida albicans |
47000 | - |
x * 47000 + x * 38000, SDS-PAGE | Candida albicans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
only partial, due to the labile nature of the enzyme further purification is unsuccessful | Candida albicans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.068 | - |
partially purified enzyme | Candida albicans |
Storage Stability | Organism |
---|---|
4°C, Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT, 1 mM UMP, loss of 40% activity in 1 week | Candida albicans |
4°C, Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT, loss of 70% activity in 4 days | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate | 1.6fold higher specific activity compared to uracil | Candida albicans | 5-fluoro-UMP + diphosphate | - |
? | |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Candida albicans | UMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000 + x * 38000, SDS-PAGE | Candida albicans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
4 min, complete loss of activity | Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Candida albicans |