Cloned (Comment) | Organism |
---|---|
three genes FucTA, FucTB and FucTC encode proteins similar to alpha1-3-fucosyltransferases, expression in Pichia pastoris | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
dansyl-GnGnF6 | glycopeptide derived from IgG, FucTA gene | Drosophila melanogaster | |
0.046 | - |
dansyl-GnGn | glycopeptide derived from IgG, FucTA gene | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine | Drosophila melanogaster | - |
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-fucose + dabsyl-GnGn-peptide | GnGn is the most suitable substrate for FucTA | Drosophila melanogaster | GDP + alpha1-3-fucosylated dabsyl-GnGn-peptide | - |
? | |
GDP-fucose + dansyl-GnGn | - |
Drosophila melanogaster | GDP + alpha1-3-fucosylated glycopeptide | - |
? | |
GDP-fucose + dansyl-GnGnF6 | prefucosylation of GnGn with chicken heart extract results in a superior conversion of GnGnF6 by FucTA than with GnGn, 100% relative conversion | Drosophila melanogaster | GDP + alpha1-3-fucosylated glycopeptide | - |
? | |
GDP-fucose + GalGal-N-glycan | 4.6% relative conversion, FucTA gene | Drosophila melanogaster | GDP + alpha1-3-fucosylated GalGal-N-glycan | - |
? | |
GDP-fucose + GalGalF6-N-glycan | 19.8% relative conversion, FucTA gene | Drosophila melanogaster | GDP + alpha1-3-fucosylated GalGalF6-N-glycan | - |
? | |
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine | - |
Drosophila melanogaster | GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine | - |
? | |
additional information | the enzyme is able to fucosylate N-glycan structures of human transferrin in vitro | Drosophila melanogaster | ? | - |
? | |
additional information | alpha1-6-fucosylated glycans are better substrates than beta-1-4-galactosylated glycans, FucTA gene | Drosophila melanogaster | ? | - |
? |