Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.213 extracted from

  • Hagemann, M.; Ribbeck-Busch, K.; Klaehn, S.; Hasse, D.; Steinbruch, R.; Berg, G.
    The plant-associated bacterium Stenotrophomonas rhizophila expresses a new enzyme for the synthesis of the compatible solute glucosylglycerol (2008), J. Bacteriol., 190, 5898-5906.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.213

Activating Compound

Activating Compound Comment Organism Structure
additional information gene ggpPS is induced by salt shock Stenotrophomonas rhizophila

Cloned(Commentary)

Cloned (Comment) Organism
gene ggpPS, DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and truncated mutant enzymes in Escherichia coli strain LMG194, complementation of the glucosylglycerol-defective mutants of the cyanobacterium Synechocystis sp. strain PCC 6803 Stenotrophomonas rhizophila

Protein Variants

Protein Variants Comment Organism
additional information construction of a truncated enzyme version devoid of the phosphatase part, which synthesizes glucosylglycerol-phosphate, in contrast to the full-length protein, that synthesizes glucosylglycerol, dephosphorylation of glucosylglycerol phosphate is detected only with the complete GgpPS protein, overview. Expression of the full-length ggpPS gene leads to complementation of the salt-sensitive phenotype of the mutant defective in the phosphatase step showing a severe drop in salt resistance because of the toxic effect of glucosylglycerol phosphate accumulation Stenotrophomonas rhizophila

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Stenotrophomonas rhizophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Stenotrophomonas rhizophila the rhizobacterium Stenotrophomonas rhizophila accumulates the compatible solutes glucosylglycerol and trehalose under salt stress conditions, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Stenotrophomonas rhizophila Q5GMP2 a plant associated bacterium, gene ggpPS
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and truncated mutant enzymes from Escherichia coli strain LMG194 by nickel affinity chromatography Stenotrophomonas rhizophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the rhizobacterium Stenotrophomonas rhizophila accumulates the compatible solutes glucosylglycerol and trehalose under salt stress conditions, overview Stenotrophomonas rhizophila ?
-
 ?
additional information a truncated enzyme version, devoid of the phosphatase part, synthesizes glucosylglycerol-phosphate, in contrast to the full-length wild-type enzyme, that synthesizes glucosylglycerol. The complete GgpPS protein also catalyzes dephosphorylation of glucosylglycerol phosphate, overview Stenotrophomonas rhizophila ?
-
 ?

Subunits

Subunits Comment Organism
More the enzyme shows a C-terminal GG-phosphate synthase domain and an N-terminal putative GG-phosphate phosphatase domain Stenotrophomonas rhizophila

Synonyms

Synonyms Comment Organism
GGPPS
-
 Stenotrophomonas rhizophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Stenotrophomonas rhizophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Stenotrophomonas rhizophila