Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Niallia circulans |
Protein Variants | Comment | Organism |
---|---|---|
D577A | the mutation increases the beta-cyclization activity of the enzyme (23% higher catalytic efficiency compared to the wild type) | Niallia circulans |
D577G | the mutation increases the beta-cyclization activity of the enzyme (43.9% higher catalytic efficiency compared to the wild type) | Niallia circulans |
D577I | the mutation decreases the beta-cyclization activity of the enzyme (14.5% lower catalytic efficiency compared to the wild type) | Niallia circulans |
D577L | the mutation decreases the beta-cyclization activity of the enzyme (8.8% lower catalytic efficiency compared to the wild type) | Niallia circulans |
D577V | the mutation decreases the beta-cyclization activity of the enzyme (18.8% lower catalytic efficiency compared to the wild type) | Niallia circulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | P43379 | - |
- |
Niallia circulans STB01 | P43379 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltodextrin + glycosyl acceptor | - |
Niallia circulans | beta-cyclodextrin + alpha-cyclodextrin + gamma-cyclodextrin | - |
? | |
maltodextrin + glycosyl acceptor | - |
Niallia circulans STB01 | beta-cyclodextrin + alpha-cyclodextrin + gamma-cyclodextrin | - |
? | |
soluble starch + glycosyl acceptor | - |
Niallia circulans | beta-cyclodextrin + alpha-cyclodextrin + gamma-cyclodextrin | - |
? | |
soluble starch + glycosyl acceptor | - |
Niallia circulans STB01 | beta-cyclodextrin + alpha-cyclodextrin + gamma-cyclodextrin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CGTase | - |
Niallia circulans |
cyclodextrin glycosyltransferase | - |
Niallia circulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
371 | - |
maltodextrin | mutant enzyme D577G, at pH 6.5 and 50°C | Niallia circulans | |
403 | - |
maltodextrin | wild type enzyme, at pH 6.5 and 50°C | Niallia circulans | |
406 | - |
maltodextrin | mutant enzyme D577A, at pH 6.5 and 50°C | Niallia circulans | |
467 | - |
maltodextrin | mutant enzyme D577V, at pH 6.5 and 50°C | Niallia circulans | |
513 | - |
maltodextrin | mutant enzyme D577I, at pH 6.5 and 50°C | Niallia circulans | |
583 | - |
maltodextrin | mutant enzyme D577L, at pH 6.5 and 50°C | Niallia circulans |