Protein Variants | Comment | Organism |
---|---|---|
additional information | MGAT1 protein and mRNA levels are markedly reduced in cells infected with lentiviral expressing MGAT1 shRNA-1 or MGAT1-shRNA-2. Knockdown of MGAT1 leads to an increase in the G1-phase cells with a decrease in S-phase cells and G2/M-phase cells. Downregulation of MGAT1 significantly inhibits migration of GBM cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | - |
Homo sapiens | 139 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P26572 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
glioblastoma cell | MGAT1 is highly expressed in glioblastoma tissues | Homo sapiens | - |
glioma cell | cell proliferation and migration analysis, overview | Homo sapiens | - |
U-251MG cell | - |
Homo sapiens | - |
U-87MG cell | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
MGAT1 | - |
Homo sapiens |
N-acetylglucosaminyltransferase I | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | downregulation of MGAT1 inhibits glioma cell proliferation and migration, overview. Glut1 protein expression is significantly decreased in MGAT1 knockdown cells, but there is no significant change in Glut3. Activation of EGFR signalling by HB-EGF can rescue the inhibitory effects of MGAT1 knockdown on the expression of Glut1, but downregulation of MGAT1 does not significantly change the level of Glut1 mRNA. Downregulation of MGAT1 decreases the complex N-glycan of Glut1. Ectopic expression of Glut1 rescues the inhibitory effects of MGAT1 knockdown on glioma cell proliferation and migration | Homo sapiens |
additional information | Glut1 N-glycan structure is evaluated by N-glycosidase digestion. Glut1 in U87/MG cells is sensitive to PNGase F but resistant to endo H digestion, suggesting that the type of Glut1 N-glycan in U87/MG cells are mainly complex type N-glycan | Homo sapiens |
physiological function | N-acetylglucosaminyltransferase I (MGAT1) is responsible for the conversion of high-mannose to hybrid and complex N-glycans. It promotes glioma cell proliferation and migration through increasing the stability of the glucose transporter GLUT1, MGAT1 interacted with Glut1 in U-87/MG cells, mechanism of MGAT1 promoting glioma cell proliferation, overview. MGAT1 regulates Glut1 N-glycosylation | Homo sapiens |