Cloned (Comment) | Organism |
---|---|
2 cytosolic HMG-CoA synthase genes, HMGS-1 and HMGs-2 described, HMGS-1 cloned in expression vector pSBLA1 and overexpressed in Escherichia coli BL21DE3 pLys S | Blattella germanica |
intronless gene, encodes for an active enzyme able to complement eukaryotic Mev-1 cells | Blattella germanica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | - |
Blattella germanica | |
acetoacetyl-CoA | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
acetoacetyl-CoA | - |
Saccharomyces cerevisiae | |
0.014 | - |
acetyl-CoA | - |
Saccharomyces cerevisiae | |
0.0152 | - |
acetyl-CoA | - |
Blattella germanica | |
0.051 | - |
acetyl-CoA | - |
Bos taurus | |
0.2 | - |
acetyl-CoA | - |
Diploptera punctata | |
0.3 | - |
acetyl-CoA | - |
Gallus gallus | |
0.4 | - |
acetyl-CoA | - |
Locusta migratoria |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Gallus gallus | 5829 | - |
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
cytosol | - |
Blattella germanica | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE, immunoblot by antibodies, prediction from cDNA nucleotide sequence | Blattella germanica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | Gallus gallus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Saccharomyces cerevisiae | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Bos taurus | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Locusta migratoria | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Diploptera punctata | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | Blattella germanica | regulation of isoprenoid synthesis and metabolism | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blattella germanica | - |
cockroach | - |
Bos taurus | - |
ox | - |
Diploptera punctata | - |
- |
- |
Gallus gallus | - |
chicken | - |
Locusta migratoria | - |
- |
- |
Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Blattella germanica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
corpus allatum | - |
Locusta migratoria | - |
corpus allatum | - |
Diploptera punctata | - |
fat body | - |
Blattella germanica | - |
liver | - |
Gallus gallus | - |
liver | - |
Bos taurus | - |
ovary | - |
Blattella germanica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Blattella germanica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Gallus gallus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Saccharomyces cerevisiae | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Bos taurus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Locusta migratoria | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Blattella germanica | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Diploptera punctata | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | regulation of isoprenoid synthesis and metabolism | Blattella germanica | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
additional information | - |
Gallus gallus | ? | - |
? | |
additional information | - |
Saccharomyces cerevisiae | ? | - |
? | |
additional information | first organism known to have 2 functional cytosolic HMG-CoA synthases | Blattella germanica | ? | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00126 | - |
acetoacetyl-CoA | - |
Blattella germanica | |
0.0035 | - |
acetoacetyl-CoA | - |
Bos taurus |