Crystallization (Comment) | Organism |
---|---|
determination of the crystal of a complex between the HECT domain NEDD4L and the E2 UbcH5B bearing a covantly-linked ubiquitin at its active site to gain insights of the ubiquitin transfer from an associated E2 to the acceptor cysteine in the HECT domain C-lobe. HECT contains is bilobed, consists of N-terminal N-lobe and C-terminal C-lobe. The C-lobe is essential for the E2-to-E3 ubiquitin transfer and the N-lobe is essential for the the further protein substrate processivity, the transfer of the ubiquitin from E3 to the substrate | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P62837 | - |
- |
Homo sapiens | Q96PU5 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine = [ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine | the 40 kDa C-terminal HET domain binds a reactive thioester-linked E2-ubiquitin covalent-linked complex. The ubiqiutin is transferred from the E2 catalytic cysteine to the HECT domain catalytic cysteine via a transthiolation reaction | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
E3 HECT | - |
Homo sapiens |
E3 ubiquitin-protein ligase | - |
Homo sapiens |
UbcH5B-ubiquitin-HECT NEDD4L complex | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | part of the E1-E2-E3 ubiquitin conjugation cascade. The E2-ubiquitin covalent complex interacts with an E3 (HECT) for ubiquitin transfer. Ubiquitin is transferred to the acceptor cysteine of the C-lobe of the HECT domain | Homo sapiens |