Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00021 | - |
L-alanyl-tRNA | pH 7.6, 37°C | Streptococcus pneumoniae | |
0.031 | - |
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine | pH 7.6, 37°C | Streptococcus pneumoniae | |
0.035 | - |
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | pH 7.6, 37°C | Streptococcus pneumoniae | |
0.037 | - |
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | pH 7.6, 37°C | Streptococcus pneumoniae | |
0.237 | - |
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine | pH 7.6, 37°C | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
penicillin-resistant strain | - |
Streptococcus pneumoniae | - |
penicillin-susceptible strain | - |
Streptococcus pneumoniae 159 | - |
penicillin-resistant strain | - |
Streptococcus pneumoniae Pn16 | - |
penicillin-susceptible strain | - |
Purification (Comment) | Organism |
---|---|
His-tagged recombinant protein | Streptococcus pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.172 | - |
substrate L-alanyl-tRNA from penicillin-susceptible strain Pn16, pH 7.6, 37°C | Streptococcus pneumoniae |
0.175 | - |
substrate L-alanyl-tRNA from penicillin-resistant strain 159, pH 7.6, 37°C | Streptococcus pneumoniae |
0.247 | - |
substrate L-seryl-tRNA from penicillin-resistant strain 159, pH 7.6, 37°C | Streptococcus pneumoniae |
0.28 | - |
substrate L-seryl-tRNA from penicillin-resistant strain 159, pH 7.6, 37°C | Streptococcus pneumoniae |
0.31 | - |
substrate L-seryl-tRNA from penicillin-susceptible strain Pn16, pH 7.6, 37°C | Streptococcus pneumoniae |
0.37 | - |
substrate L-seryl-tRNA from penicillin-susceptible strain Pn16, pH 7.6, 37°C | Streptococcus pneumoniae |
1.92 | - |
substrate L-alanyl-tRNA from penicillin-resistant strain 159, pH 7.6, 37°C | Streptococcus pneumoniae |
2.09 | - |
substrate L-alanyl-tRNA from penicillin-susceptible strain Pn16, pH 7.6, 37°C | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA | i.e. lipid I | Streptococcus pneumoniae | ? + tRNA | - |
? | |
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA | i.e. lipid I | Streptococcus pneumoniae 159 | ? + tRNA | - |
? | |
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA | i.e. lipid I | Streptococcus pneumoniae Pn16 | ? + tRNA | - |
? | |
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine + alanyl-tRNA | - |
Streptococcus pneumoniae | ? + tRNA | - |
? | |
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine + alanyl-tRNA | - |
Streptococcus pneumoniae 159 | ? + tRNA | - |
? | |
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine + alanyl-tRNA | - |
Streptococcus pneumoniae Pn16 | ? + tRNA | - |
? | |
additional information | differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla | Streptococcus pneumoniae | ? | - |
? | |
additional information | MurM acts during the lipid-linked stages of peptidoglycan synthesis.The D-alanyl-D-alanine of the stem peptide and the lipid II N-acetylglucosaminyl group are not essential for substrate recognition. epsilon-Carboxylation of the lysine of the stem peptide is not tolerated. Differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla | Streptococcus pneumoniae | ? | - |
? | |
additional information | differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla | Streptococcus pneumoniae 159 | ? | - |
? | |
additional information | MurM acts during the lipid-linked stages of peptidoglycan synthesis.The D-alanyl-D-alanine of the stem peptide and the lipid II N-acetylglucosaminyl group are not essential for substrate recognition. epsilon-Carboxylation of the lysine of the stem peptide is not tolerated. Differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla | Streptococcus pneumoniae 159 | ? | - |
? | |
additional information | differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla | Streptococcus pneumoniae Pn16 | ? | - |
? | |
additional information | MurM acts during the lipid-linked stages of peptidoglycan synthesis.The D-alanyl-D-alanine of the stem peptide and the lipid II N-acetylglucosaminyl group are not essential for substrate recognition. epsilon-Carboxylation of the lysine of the stem peptide is not tolerated. Differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla | Streptococcus pneumoniae Pn16 | ? | - |
? | |
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA | in vivo and in vitro, in the presence of the appropriate acyl-tRNA, MurM alanylates the peptidoglycan-amino group of the stem peptide lysine in preference to its serylation | Streptococcus pneumoniae | UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA | - |
? | |
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA | MurMPn16 of penicillin-susceptible strain Pn16 supports serylation more than alanylation both in vivo and in vitro | Streptococcus pneumoniae | UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA | - |
? | |
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA | in vivo and in vitro, in the presence of the appropriate acyl-tRNA, MurM alanylates the peptidoglycan-amino group of the stem peptide lysine in preference to its serylation | Streptococcus pneumoniae 159 | UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA | - |
? | |
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA | in vivo and in vitro, in the presence of the appropriate acyl-tRNA, MurM alanylates the peptidoglycan-amino group of the stem peptide lysine in preference to its serylation | Streptococcus pneumoniae Pn16 | UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA | - |
? | |
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-seryl-tRNA | in vivo and in vitro, in the presence of the appropriate acyl-tRNA, MurM alanylates the peptidoglycan-amino group of the stem peptide lysine in preference to its serylation | Streptococcus pneumoniae | UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + tRNA | - |
? | |
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-seryl-tRNA | MurMPn16 of penicillin-susceptible strain Pn16 supports serylation more than alanylation both in vivo and in vitro | Streptococcus pneumoniae | UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + tRNA | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine | pH 7.6, 37°C | Streptococcus pneumoniae | |
0.0157 | - |
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine | pH 7.6, 37°C | Streptococcus pneumoniae | |
0.017 | - |
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | pH 7.6, 37°C | Streptococcus pneumoniae | |
0.021 | - |
L-alanyl-tRNA | pH 7.6, 37°C | Streptococcus pneumoniae | |
0.033 | - |
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | pH 7.6, 37°C | Streptococcus pneumoniae |
General Information | Comment | Organism |
---|---|---|
physiological function | adds L-serine or L-alanine as the first amino acid of a dipeptide branch to the stem peptide lysine of the pneumococcal peptidoglycan. MurM activity is essential for clinical pneumococcal penicillin resistance | Streptococcus pneumoniae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine | pH 7.6, 37°C | Streptococcus pneumoniae | |
0.1 | - |
L-alanyl-tRNA | pH 7.6, 37°C | Streptococcus pneumoniae | |
0.205 | - |
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | pH 7.6, 37°C | Streptococcus pneumoniae | |
0.46 | - |
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine | pH 7.6, 37°C | Streptococcus pneumoniae | |
0.948 | - |
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | pH 7.6, 37°C | Streptococcus pneumoniae |