Protein Variants | Comment | Organism |
---|---|---|
K118R | residue Lys118 is required for Ubc7 activity. Mutant is very poor in assembly of polyubiquitin chains. Lys118 is both essential and sufficient for Doa10-mediated degradation of substrates | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Saccharomyces cerevisiae | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P40318 | - |
- |
Saccharomyces cerevisiae | Q08109 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
Der3 | - |
Saccharomyces cerevisiae |
ERAD-associated E3 ubiquitin-protein ligase DOA10 | - |
Saccharomyces cerevisiae |
ERAD-associated E3 ubiquitin-protein ligase HRD1 | - |
Saccharomyces cerevisiae |
SSM4 | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | in vivo degradation of substrates for integral endoplasmic reticulum membrane E3 ubiquitin ligase Doa10 is inhibitied by mutations in the alpha2 helix of E2 enzyme Ubc7 by preventing the conjugation of donor ubiquitin to the acceptor. Ubiquitin chain formation by mutant Ubc7 is not restored by the E3 enzyme Doa10 RING domain. alpha2 Helix mutations selectively impair the in vivo degradation of Doa10 substrates | Saccharomyces cerevisiae |
physiological function | in vivo degradation of substrates for integral endoplasmic reticulum membrane E3 ubiquitin ligase Hrd1 is not impaired by mutations within helix alpha2 of ubiquitin-conjugating enzyme Ubc7. Ubiquitin-chain formation by mutant Ubc7 is restored selectively by the E3 enzyme Hrd1 RING domain | Saccharomyces cerevisiae |