Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Cavia porcellus | |
Cu2+ | weak | Streptomyces sp. | |
monoiodoacetate | 1 mM, 97% inhibition | Cavia porcellus | |
monoiodoacetate | 1 mM, 24% inhibition | Streptomyces sp. | |
additional information | - |
Cavia porcellus | |
additional information | not inhibited by Ba2+, Co2+, Fe3+, K+, Mg2+, Mn2+, Na+, Ni2+, Sr2+ | Streptomyces sp. | |
N-ethylmaleimide | 1 mM, 75% inhibition | Cavia porcellus | |
N-ethylmaleimide | 1 mM, 90% inhibition | Streptomyces sp. | |
p-chloromercuribenzoate | 1 mM, 98% inhibition | Cavia porcellus | |
p-chloromercuribenzoate | 1 mM, 44% inhibition | Streptomyces sp. | |
Pb(CH3COO)2 | 1 mM, 56% inhibition | Streptomyces sp. | |
Zn2+ | - |
Cavia porcellus | |
Zn2+ | 1 mM, 89% inhibition | Streptomyces sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces sp. | - |
- |
soluble | - |
Cavia porcellus | - |
- |
soluble | - |
Streptomyces sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by Ca2+ | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Streptomyces sp. | - |
tentatively classified, strain S-8112 | - |
Purification (Comment) | Organism |
---|---|
amberlite CG-50, Blue Sepharose | Streptomyces sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Streptomyces sp. | - |
culture filtrate | - |
Streptomyces sp. | - |
liver | - |
Cavia porcellus | - |
liver | - |
Streptomyces sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22.6 | - |
- |
Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + carbobenzoxy-Gln-Gly | other substrates are carbobenzoxy-Gln-Gln-Gly, carbobenzoxy-Gly-Gln-Gln-Gly, carbobenzoxy-Gly-Gly-Gln-Gly with 38%, 13% and 28% efficiency, respectively | Streptomyces sp. | carbobenzoxy-Gln-Gly-hydroxamate + ? | - |
? | |
protein-bound gamma-glutamine + alkylamine | - |
Cavia porcellus | protein N5-alkylglutamine + NH3 | - |
? | |
protein-bound gamma-glutamine + alkylamine | no activity with native bovine serum albumin | Streptomyces sp. | protein N5-alkylglutamine + NH3 | - |
? | |
protein-bound gamma-glutamine + alkylamine | no activity with bovine myosin, histone mixture, human serum fibronectin, spinach ribulose 1,5-diphosphate carboxylase-oxygenase, carbobenzoxyglutamine, carbobenzoxy-Asn-Gly | Streptomyces sp. | protein N5-alkylglutamine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Cavia porcellus |
monomer | 1 * 40000, SDS-PAGE | Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cavia porcellus |
37 | - |
assay at | Streptomyces sp. |
50 | - |
at pH 6.0 | Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
10 min stable, pH 5-9 | Streptomyces sp. |
40 | - |
10 min stable, pH 7 | Streptomyces sp. |
50 | - |
26% loss of activity within 10 min, pH 7.0 | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Cavia porcellus |
additional information | - |
pI: 8.9 | Streptomyces sp. |
6 | 7 | - |
Streptomyces sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | 10 min stable, 37°C | Streptomyces sp. |